IFN gamma Rat anti-Mouse, Functional Grade, Clone: AN-18, eBioscience™
Rat Monoclonal Antibody
Manufacturer: Invitrogen 16731385
Description: The AN-18 antibody reacts with mouse interferon-gamma (IFN gamma), a 15-20 kDa cytokine secreted by Th1 cells. Applications Reported: The AN-18 antibody has been reported for use in ELISA and ELISPOT assay. For neutralization of IFN gamma activity, the alternate clones R4-6A2 or XMG1.2 are recommended. Applications Tested: The AN-18 antibody has been tested as the capture antibody in IFN gamma ELISPOT assay, in combination with the biotinylated R4-6A2 antibody for detection. A concentration of 1-4 µg/mL is recommended. For neutralization of IFN gamma activity, the alternate clones R4-6A2 or XMG1.2 are recommended. Endotoxin: Less than 0.001 ng/ug antibody as determined by the LAL assay. Storage and handling: Use in a sterile environment. Filtration: 0.2 μm post-manufacturing filtered. Purity: Greater than 90%, as determined by SDS-PAGE. Endotoxin Level: Less than 0.001 ng/μg antibody, as determined by LAL assay. Aggregation: Less than 10%, as determined by HPLC.IFN gamma (Interferon gamma, Type II interferon) is a macrophage activation factor, and immune interferon that is produced primarily by T-lymphocytes and natural killer cells in response to antigens, mitogens, Staphylococcus enterotoxin B, phytohemaglutanin and other cytokines. IFN gamma is a dimeric protein consisting of two 146 amino acid subunits. IFN gamma is a glycoprotein that exists, functionally, as a homodimer of approximately 45 kDa. On SDS-PAGE, IFN gamma appears as a combination of 25, 20 and minor 15.5 kDa bands as a result of differential glycosylation. The biological activity of the IFN gamma homodimer is highly species specific. Human IFN gamma does not show cross-reactivity with mouse. IFN gamma function includes the following: antiviral activity, tumor antiproliferative activity, induction of class I and II MHC, macrophage activation, and enhanced immunoglobulin secretion by B lymphocytes. IFN gamma is involved in cytokine regulation and also acts synergistically with other cytokines. Activation of IFN gamma takes place through binding of IFN gamma receptor I and II, and activating the JAK-STAT pathway. IFN gamma does not show any homology with IFN alpha or IFN beta but human IFN gamma shows about 40% sequence homology with mouse IFN gamma. IFN gamma is upregulated by IL2, FGF basic, EGF and downregulated by vitamin D3 or DMN. IFN gamma gene mutations are associated with aplastic anemia.
|PBS with no preservative; pH 7.2|
|Control, ELISA, Functional Assay|
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