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Kappa Light Chain Mouse anti-Human, eFluor® 450, Clone: TB28-2, eBioscience™ Non-distribution product as customer accommodation.

Mouse Monoclonal Antibody

$139.00 - $322.00

Specifications

Antigen Kappa Light Chain
Clone TB28-2
Target Species Human
Conjugate eFluor™ 450
Isotype IgG1, kappa
View More Specs

Products 2
Catalog Number Mfr. No. Quantity Price Quantity & Availability  
Catalog Number Mfr. No. Quantity Price Quantity & Availability  
50-164-21 Non-distribution product as customer accommodation. View Documents Invitrogen
48997041
25 Tests Each for $139.00
Add to cart
 
50-164-22 Non-distribution product as customer accommodation. View Documents Invitrogen
48997042
100 Tests Each for $322.00
Add to cart
 
Description

Description

Description: The monoclonal antibody TB28-2 recognizes the kappa light chain of human immunoglobulin. Approximately 22-kDa, the Ig kappa chain is comprised of one variable and one constant region. Two kappa chains together with the heavy chain comprise the whole Ig structure. Expression of Ig kappa is typically found on more than half of B lymphocytes. Applications Reported: This TB28-2 antibody has been reported for use in flow cytometric analysis. Applications Tested: This TB28-2 antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 µL (0.5 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. eFluor™ 450 is an alternative to Pacific Blue™. eFluor™ 450 emits at 445 nm and is excited with the Violet laser (405 nm). Please make sure that your instrument is capable of detecting this fluorochome. Excitation: 405 nm; Emission: 445 nm; Laser: Violet Laser. Filtration: 0.2 µm post-manufacturing filtered.

Immunoglobulin classes share the same basic four polypeptide chain structure of two heavy chains (five types) and two light chains (kappa, lambda; both having a molecular weight of 22.5 kDa). Kappa and lambda consist of a variable region and a constant region and can easily be differentiated by the antigenic properties of the constant region. The ratio of kappa to lambda is 70:30. Antibodies to the kappa light chain are reportedly useful in the identification of leukemias, plasmacytomas, and certain non-Hodgkin ft.s lymphomas. Demonstration of clonality in lymphoid infiltrates indicates that the infiltrate is clonal and therefore maligt.
Specifications

Specifications

Kappa Light Chain
Human
IgG1, kappa
Monoclonal
Liquid
Antibody
4° C, store in dark, DO NOT FREEZE!
TB28-2
eFluor™ 450
Flow Cytometry
Primary
Monoclonal
PBS with 0.1% gelatin, 0.2% BSA and 0.09% sodium azide; pH 7.2
RUO

For Research Use Only.

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