Invitrogen™ Human IgA ELISA Kit

Description

ELISA

The Human ImmunoGlobulin A (Hu IgA) ELISA quantitates Hu IgA in human serum or plasma. The assay will exclusively recognize both natural and recombinant Hu IgA. Principle of the method The Human IgA solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

Human IgA, also known as human immunoglobulin A, is an antibody that is produced by the immune system in response to antigens. It is the most abundant immunoglobulin in mucosal secretions, such as saliva, tears, and breast milk, and plays a key role in defense against pathogens at mucosal surfaces. IgA works by binding to specific antigens and preventing them from entering the body's tissues. It can also activate complement and promote phagocytosis by immune cells. Human IgA can be used in research to study the immune response to pathogens and to develop diagnostic tests and therapeutic interventions for infectious diseases.

Specifications

• Assay Range: 1.6 to 100 ng/mL
• Assay Sensitivity: 0.03 ng/mL
• Conjugate: HRP
• Product Type: ELISA
• Sample Type: Plasma, Serum
• Target Species: Human
• For Use With (Application): ELISA
• For Use With (Equipment): Colorimetric Microplate Reader
• Gene ID (Entrez): 3493, 3494
• Interassay CV: 4.8%
• Intraassay CV: 3.9
• Kit Contents: Pre-coated 96 well plate, Standard, Sample Diluent, Assay Buffer concentrate, HRP-conjugated Detection Antibody, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers
• Label or Dye: HRP
• Quantity: 10 x 96 Tests
• Regulatory Status: RUO
• Sample Volume: Plasma 10 μL, Serum 10 μL
• Storage Requirements: 2°C to 8°C
• Synonym: Immunoglobulin A
• Target: Human IgA
• Test Time: 1 hrs 20 mins
• Total Assay Time: 1 hrs 30 mins