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IgD Mouse anti-Mouse, Functional Grade, Clone: 10.4.22, eBioscience™ Non-distribution product as customer accommodation.

Mouse Monoclonal Antibody

Manufacturer:  Invitrogen 16592485

Catalog No. 50-112-8593


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Description

Description

Description: This 10.4.22 monoclonal antibody reacts with the a allotype of mouse IgD, which is expressed by mature peripheral B cells. This antibody has been reported to activate B cells via engagement of the IgD receptor. The use of anti-IgM as a polyclonal B cell activator is problematic due to interference from high levels of circulating serum IgM. Crossblocking studies suggest that the 10.4.22 antibody recognizes a different epitope from 11-26c. Applications Reported: This 10.4.22 antibody has been reported for use in functional assays. Applications Tested: This 10.4.22 antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 0.25 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Endotoxin: Less than 0.001 ng/ug antibody as determined by the LAL assay. Storage and handling: Use in a sterile environment. Filtration: 0.2 µm post-manufacturing filtered. Purity: Greater than 90%, as determined by SDS-PAGE. Endotoxin Level: Less than 0.001 ng/µg antibody, as determined by LAL assay. Aggregation: Less than 10%, as determined by HPLC.

This type I transmembrane protein is co-expressed on the surface of mature naive B cells with membrane IgM. IgD also exists as a soluble form in blood serum. Studies have demonstrated that IgD associates with the B cell receptor and participates in mediating signal transduction upon antigen binding.
Specifications

Specifications

IgD
Monoclonal
1 mg/mL
PBS with no preservative; pH 7.2
Liquid
Mouse
Affinity chromatography
RUO
Antibody
Monoclonal
Mouse
Functional Assay
10.4.22
Functional Grade
Ighd
Ighd
IgG2a, kappa
500 μg
4° C
Primary
380797
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