IgD Rat anti-Mouse, PE, Clone: 11-26c (11-26), eBioscience
Rat Monoclonal Antibody
Manufacturer: Invitrogen 12599382
Description: The 11-26c monoclonal antibody reacts with the delta heavy chain of mouse IgD. It does not react with other classes of mouse immunoglobulin including IgA, IgG or IgM. IgD is expressed by peripheral mature B cells. 11-26c does not activate B cells. Applications Reported: The 11-26c (11-26) antibody has been reported for use in flow cytometric analysis to detect the surface IgD on mouse B cells. Applications Tested: The 11-26c (11-26) antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 0.125 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser. Filtration: 0.2 µm post-manufacturing filtered.This type I transmembrane protein is co-expressed on the surface of mature naive B cells with membrane IgM. IgD also exists as a soluble form in blood serum. Studies have demonstrated that IgD associates with the B cell receptor and participates in mediating signal transduction upon antigen binding.
|PBS with 0.1% gelatin and 0.09% sodium azide; pH 7.2|
|4° C, store in dark, DO NOT FREEZE!|
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