Pig anti-Human IgE Fc Secondary Antibody, Novus Biologicals™
Pig Polyclonal Antibody
Supplier: Novus Biologicals NBP269114
DescriptionIgE Fc Polyclonal specifically detects IgE Fc in Human samples. It is validated for ELISA, Immunohistochemistry, Immunocytochemistry/Immunofluorescence, Immunohistochemistry-Paraffin, Immunoblotting, Dot Blot.
|Lyophilized from PBS (pH 7.2) with No Preservative|
|Purified homogenous IgE isolated from human serum. Freund's complete adjuvant is used in the first step of the immunization procedure.|
|The reactivity of the antiserum is directed to the Fc subunits of the IgE molecule which represents strict isotype (class) specificity. In immunoelectrophoresis and double radial immunodiffusion, using various antiserum concentrations against appropriate concentrations of IgE, a single precipitin line is obtained which shows a reaction of full identity with the precipitin line obtained with the used immunogen. It does not react with human IgG, including all subclasses, IgA, IgM and IgG/Fab or any non-Ig protein in human serum.|
|ELISA, Immunocytochemistry, Immunofluorescence, Immunohistochemistry (Paraffin), Immunoblot, Dot Blot|
|ELISA 1:100-1:2000, Immunocytochemistry/Immunofluorescence 1:10 - 1:500, Immunohistochemistry-Paraffin 1:10 - 1:500, Immunoblotting 1:100 - 1:2000, Dot Blot 1:100-1:2000|
|Ion exchange chromatography|
|Reconstitute by by adding 1 ml sterile distilled water, spun down to remove insoluble particles, divided into small aliquots, frozen and stored at or below -20C.|
|Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.|
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