IgE Mouse anti-Human, PE-Cyanine7, Clone: Ige21, eBioscience
Mouse Monoclonal Antibody
Manufacturer: Invitrogen 25698642
Description: The monoclonal antibody IgE21 recognizes the immunoglobulin E class of antibodies. The natural levels of IgE in the serum are quite low, comprising only a small portion of the total immunoglobulin. IgE plays a role in allergy responses by binding to mast cells and basophils through the Fc epsilon receptor I which results in the release of histamine and other molecules. The IgE is produced by terminally differentiated plasma B cells and basophils. Like all immunoglobulins, IgE is expressed as a soluble molecule as well as a membrane bound form. IgE21 recognizes both forms. Applications Reported: This Ige21 antibody has been reported for use in flow cytometric analysis. Applications Tested: This Ige21 antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 µL (0.06 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. Light sensitivity: This tandem dye is sensitive to photo-induced oxidation. Please protect this vial and stained samples from light. Fixation: Samples can be stored in IC Fixation Buffer (cat. 00-8222) (100 µL of cell sample + 100 µL of IC Fixation Buffer) or 1-step Fix/Lyse Solution (cat.00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically. Excitation: 488-561 nm; Emission: 775 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser. Filtration: 0.2 µm post-manufacturing filtered. IgE is one of the five classes of immunoglobulins found in mammals. Monomers consist of two heavy (epsilon) chains with 4 Ig-like constant domains, and two light chains. IgE plays an essential role in type I hypersensitivity (allergic responses such as hay fever, asthma, hives, anaphylatic shock) by binding to Fc receptors on basophils and mast cells. Crosslinking of IgE bound to Fc receptors induces degranulation of mast cells and basophils that promote allergic manifestations. IgE is also functionally important for immunity against parasites.
|PBS with 0.1% gelatin, 0.2% BSA and 0.09% sodium azide; pH 7.2|
|4° C, store in dark, DO NOT FREEZE!|
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