Promotional price valid on web orders only. Your contract pricing may differ. Interested in signing up for a dedicated account number?
Learn More

Invitrogen™ IGF1R Human ELISA Kit

Sandwich ELISA Kit

Manufacturer:  Invitrogen™ EHIGF1R

Encompass

 View more versions of this product

Catalog No. EHIGF1R


Add to cart

Description

Description

The Human Insulin-like Growth Factor 1R (Hu IGF1R) ELISA quantitates Hu IGF1R in human serum, plasma, or cell culture medium. The assay will exclusively recognize both natural and recombinant Hu IGF1R. Principle of the method The Human IGF1R solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

Insulin growth factor 1 receptor belongs to the family of tyrosine receptor kinases. It is involved in activation of various substrates including IGF and neurotensin, regulation of metabolism, cell division, maintenance, and inflammation response. The protein is a tetramer consisting of two alpha, and two beta subunits. The alpha-subunits binds to insulin growth factor 1 with higher affinity than to insulin growth factor 2. Binding results in a conformational change, followed by autophosphorylation of IGF-1R at positions 1134, 1135, 1136. The activated IGF-1R in turn, causes phosphorylation of substrate proteins, followed by sequential activation of RAS, RAF, and mitogen-activated protein kinase isoforms ERK, p38, and JNK, leading to the transcription of genes that drive proliferation. This antibody recognizes the beta-subunit of IGF-1R.
Specifications

Specifications

This ELISA kit shows no cross-reactivity with any of the cytokines tested: Human Angiogenin, BDNF, BLC, ENA-78, FGF-4, IL-1 alpha, IL-1 beta, IL-2, IL-3, IL-4, IL-5, IL-7, IL-8, IL-9, IL-10, IL-11, IL-12 p70, IL-12 p40, IL-13, IL-15, IL-309, IP-10, G-CSF, GM-CSF, IFN-gamma, Leptin, MCP-1, MCP-2, MCP-3, MDC, MIP-1 alpha, MIP-1 beta, MIP-1 delta, PARC, PDGF, RANTES, SCF, TARC, TGF-beta, TIMP-1, TIMP-2, TNF-alpha, TNF-beta, TPO, VEGF.
Colorimetric Microplate Reader
Solid-phase sandwich ELISA
Hormones & Receptors
P08069
6pg/mL to 6000pg/mL
3480
RUO
2°C to 8°C
4 hr. 45 min.
Signal Transduction
<12%
96 Tests
Plasma, Serum, Supernatant
CD221,IGFIR,IGFR,JTK13
96 assays
ELISA Kit
Biotin
HRP
8.23-6000 pg/mL
ELISA, Protein Assays, Protein Biology, Cancer Biology, Immunology, Neuroscience, Neurobiology, Protein Detection, Protein Analysis
Pre-coated 96 well plate, Standard, Assay Diluent concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers
Plasma, 50 μL; Serum, 50 μL; Supernatant, 100 μL
1 hr. 20 min.
Human
CD221, IGFIR, IGFR, JTK13
<10%
Human
6 pg/mL
SDS
Documents
Product Certifications
Promotions

Promotions

Provide Content Correction

The Fisher Scientific Encompass Program offers items which are not part of our distribution portfolio. These products typically do not have pictures or detailed descriptions. However, we are committed to improving your shopping experience. Please use the form below to provide feedback related to the content on this product.

Product Title

By clicking Submit, you acknowledge that you may be contacted by Fisher Scientific in regards to the feedback you have provided in this form. We will not share your information for any other purposes. All contact information provided shall also be maintained in accordance with our Privacy Policy.

Cancel Submit