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IgG Fc Goat anti-Rat, DyLight 550, Polyclonal, Novus Biologicals™

Goat Polyclonal Antibody

Manufacturer:  Novus Biologicals NBP261920R

Catalog No. NBP261920R


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Description

Description

IgG Fc Polyclonal antibody specifically detects IgG Fc in Rat samples. It is validated for Flow Cytometry, Immunohistochemistry, Immunofluorescence, Immunocytochemistry.
Specifications

Specifications

IgG Fc fragment
0.5 mg/mL
Flow Cytometry 1:50 - 1:200, Immunohistochemistry 1:50 - 1:500, Immunofluorescence 1:50 - 1:500, Immunocytochemistry 1:50 - 1:500
Affinity Purified
Rat IgG-Fc Fragment
Immunogen affinity purified
Adaptive Immunity
Secondary
Antiserum was solid phase adsorbed to ensure class specificity. Antiserum was cross adsorbed using human and mouse immunosorbents to remove cross reactive antibodies. The antibody to rat IgG was isolated by affinity chromatography using antigen coupled to agarose beads. F(ab')2 fragments were generated using a pepsin digestion. Fc fragments and whole IgG molecules have been removed. Fragments were conjugated to DyLight 550. Antibody concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG. By immunoelectrophoresis and ELISA this antibody reacts specifically with rat IgG. Cross reactivity with IgA and IgM is negligible. No antibody was detected against non-immunoglobulin serum proteins. Less than 1% cross reactivity to human and mouse IgG was detected. This antibody may cross react with IgG from other species.
Flow Cytometry, Immunocytochemistry, Immunofluorescence, Immunohistochemistry
DyLight 550
PBS containing 0.2% BSA with 0.09% Sodium Azide
Goat
IgG
1 ml
Store at 4C. Do not freeze.
Polyclonal
Rat
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