IgG Fc Goat anti-Rat, FITC, Polyclonal, Novus Biologicals™

Goat Polyclonal Antibody

Manufacturer:  Novus Biologicals NBP261920F

Catalog No. NBP261920F

Description

IgG Fc Polyclonal antibody specifically detects IgG Fc in Rat samples. It is validated for Flow Cytometry, Immunohistochemistry, Immunofluorescence, Immunocytochemistry.

Specifications

• Antigen: IgG Fc fragment
• Concentration: 0.5 mg/mL
• Dilution: Flow Cytometry 1:50 - 1:200, Immunohistochemistry 1:50 - 1:500, Immunofluorescence 1:50 - 1:500, Immunocytochemistry 1:50 - 1:500
• Format: Affinity Purified
• Immunogen: Rat IgG-Fc Fragment
• Purification Method: Immunogen affinity purified
• Research Discipline: Adaptive Immunity
• Primary or Secondary: Secondary
• Test Specificity: Antiserum was solid phase adsorbed to ensure class specificity. Antiserum was cross adsorbed using human and mouse immunosorbents to remove cross reactive antibodies. The antibody to rat IgG was isolated by affinity chromatography using antigen coupled to agarose beads. F(ab')2 fragments were generated using a pepsin digestion. Fc fragments and whole IgG molecules have been removed. Fragments were conjugated to fluorescein isothiocyanate (FITC). Antibody concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG. By immunoelectrophoresis and ELISA this antibody reacts specifically with rat IgG. Cross reactivity with IgA and IgM is negligible. No antibody was detected against non-immunoglobulin serum proteins. Less than 1% cross reactivity to human and mouse IgG was detected. This antibody may cross react with IgG from other species.

• Applications: Flow Cytometry, Immunocytochemistry, Immunofluorescence, Immunohistochemistry
• Conjugate: FITC
• Formulation: PBS containing 0.2% BSA with 0.09% Sodium Azide
• Host Species: Goat
• Isotype: IgG
• Quantity: 1 ml
• Storage Requirements: Store at 4C. Do not freeze.
• Monoclonal or Polyclonal: Polyclonal
• Target Species: Rat