IgG Fc Goat anti-Rat, HRP, Polyclonal, Novus Biologicals
Goat Polyclonal Antibody
Manufacturer: Novus Biologicals NBP261920H
DescriptionIgG Fc Polyclonal antibody specifically detects IgG Fc in Rat samples. It is validated for Western Blot, ELISA, Immunohistochemistry, Immunocytochemistry.
|IgG Fc fragment|
|Western Blot 1:2000 - 1:20000, ELISA 1:10000 - 1:100000, Immunohistochemistry 1:200 - 1:2000, Immunocytochemistry 1:200 - 1:2000|
|Rat IgG-Fc Fragment|
|Immunogen affinity purified|
|Antiserum was solid phase adsorbed to ensure class specificity. Antiserum was cross adsorbed using human and mouse immunosorbents to remove cross reactive antibodies. The antibody to rat IgG was isolated by affinity chromatography using antigen coupled to agarose beads. F(ab')2 fragments were generated using a pepsin digestion. Fc fragments and whole IgG molecules have been removed. Fragments were conjugated to horseradish peroxidase (HRP). Antibody concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG. By immunoelectrophoresis and ELISA this antibody reacts specifically with rat IgG. Cross reactivity with IgA and IgM is negligible. No antibody was detected against non-immunoglobulin serum proteins. Less than 1% cross reactivity to human and mouse IgG was detected. This antibody may cross react with IgG from other species.|
|ELISA, Immunocytochemistry, Immunohistochemistry, Western Blot|
|PBS containing 0.2% BSA with 0.05% Pro-Clean 400|
|Store at 4C. Do not freeze.|
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