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IgG Fc fragment Goat anti-Rat, Polyclonal, Secondary Antibody, Novus Biologicals™

Goat Polyclonal Antibody

Manufacturer:  Novus Biologicals NBP261920

Catalog No. NBP261920


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Description

Description

IgG Fc fragment Polyclonal antibody specifically detects IgG Fc fragment in Rat samples. It is validated for Western Blot, ELISA, Immunohistochemistry, Immunocytochemistry.
Specifications

Specifications

IgG Fc fragment
0.5 mg/mL
Western Blot 1:1000 - 1:20000, ELISA 1:1000 - 1:20000, Immunohistochemistry 1:200 - 1:2000, Immunocytochemistry 1:200 - 1:2000
PBS containing 0.2% BSA with 0.09% Sodium Azide
Rat IgG-Fc Fragment
Polyclonal
Immunogen affinity purified
Adaptive Immunity
Rat
Western Blot,ELISA,Immunohistochemistry,Immunocytochemistry
Unconjugated
Affinity Purified
Goat
IgG
Secondary
1 ml
Store at 4C. Do not freeze.
Antiserum was solid phase adsorbed to ensure class specificity. Antiserum was cross adsorbed using human and mouse immunosorbents to remove cross reactive antibodies. The antibody to rat IgG was isolated by affinity chromatography using antigen coupled to agarose beads. F(ab')2 fragments were generated using a pepsin digestion. Fc fragments and whole IgG molecules have been removed. Antibody concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG. By immunoelectrophoresis and ELISA this antibody reacts specifically with rat IgG. Cross reactivity with IgA and IgM is negligible. No antibody was detected against non-immunoglobulin serum proteins. Less than 1% cross reactivity to human and mouse IgG was detected. This antibody may cross react with IgG from other species.
SDS
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