Syrian Hamster IgG, PE, Isotype Control, eBioscience
Syrian Hamster Isotype Control
Manufacturer: Invitrogen 12491481
The isotype of a primary antibody and the application it is being used in can result in background staining. Primary antibody background noise can be caused by binding to Fc receptors on target cells; by non-specific interactions with cellular proteins, carbohydrates, and lipids; or by cell autofluorescence. Isotype control antibodies can act as negative controls to help differentiate non-specific background signal from specific antibody signal because they have no relevant specificity to a target antigen. While isotype controls are most commonly used in flow cytometry, they are useful in other applications such as chromatin immunoprecipitation (ChIP), immunohistochemistry, and gel shifts. Isotype controls should match with the primary antibody species and isotype so that the level of specific staining by the primary antibody may be accurately determined. If using directly labeled primary antibodies, the isotype control works best if conjugated with the same label as the test antibody.Description: This hamster IgG fraction was purified by affinity chromatography from normal Golden Syrian hamster serum.Applications Reported: Golden Syrian hamster IgG isotype control has been reported for use in flow cytometric analysis.Applications Tested: This Golden Syrian hamster IgG isotype control has been tested by flow cytometric analysis of mouse splenocyte suspension and should be used atsame concentration as experimental antibody.Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.Filtration: 0.2µm post-manufacturing filtered.
|Syrian Hamster IgG|
|Control, Flow Cytometry|
|PBS with 0.1% gelatin and 0.09% sodium azide; pH 7.2|
|Store at 2-8°C. Do not freeze. Light-sensitive material.|
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For Research Use Only.