IgG1 Mouse anti-Rat, eFluor® 615, eBioscience
Mouse Monoclonal Secondary Antibody
Manufacturer: Invitrogen 42481282
Description: The R1-12D10 monoclonal antibody recognizes rat IgG1 antibodies and can be used as a second step reagent in microscopy. The monoclonal does not recognize other rat isotypes nor does it crossreact to mouse IgG1. Applications Reported: This R1-12D10 antibody has been reported for use in microscopy, immunohistochemical staining, and immunocytochemistry. Applications Tested: This R1-12D10 antibody has been tested by immunohistochemistry on frozen mouse tissue and can be used at less than or equal to 10 µg/mL. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Filter Recommendation: When using this eFluor® 615 antibody conjugate, we recommend a filter that will capture the 615 emission wavelength (for example, Excitation 560/55, 585LP, Emission 645/75). A standard Alexa Fluor® 594 filter is acceptable. Excitation: 595 nm; Emission: 615 nm. Filtration: 0.2 µm post-manufacturing filtered.Secreted immunoglobulins (Ig) have two separable functions: one is to bind specifically to pathogenic molecules, the other is to recruit cells and molecules to destroy the opsonized pathogen.
|PBS with 0.1% gelatin and 0.09% sodium azide; pH 7.2|
|Immunocytochemistry, Immunofluorescence, Immunohistochemistry (Frozen), Immunohistochemistry (Paraffin)|
|4° C, store in dark, DO NOT FREEZE!|
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