Mouse IgG1 kappa, Alexa Fluor 488, clone: P3.6.2.8.1, Isotype Control, eBioscience™ 

Mouse Isotype Control

Manufacturer:  Invitrogen 53471441

Catalog No. 50-167-47

Description

The isotype of a primary antibody and the application it is being used in can result in background staining. Primary antibody background noise can be caused by binding to Fc receptors on target cells; by non-specific interactions with cellular proteins, carbohydrates, and lipids; or by cell autofluorescence. Isotype control antibodies can act as negative controls to help differentiate non-specific background signal from specific antibody signal because they have no relevant specificity to a target antigen. While isotype controls are most commonly used in flow cytometry, they are useful in other applications such as chromatin immunoprecipitation (ChIP), immunohistochemistry, and gel shifts. Isotype controls should match with the primary antibody species and isotype so that the level of specific staining by the primary antibody may be accurately determined. If using directly labeled primary antibodies, the isotype control works best if conjugated with the same label as the test antibody.

Description: The monoclonal mouse IgG1 K immunoglobulin is useful as an isotype control.Applications Reported: Alexa Fluor™ 488 Mouse IgG1, K Isotype Control has been reported for use in immunocytochemistry, immunohistochemistry, and flow cytometric analysis.Applications Tested: This Mouse IgG1 K Isotype Control is offered in 2 formats: - µg size: has been tested. This can be used at at the same concentration as the experimental antibody. - test size: has been pre-titrated and tested. This can be used at test size: 5 µL (1 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.Excitation: 488 nm; Emission: 519 nm; Laser: Blue Laser.Filtration: 0.2µm post-manufacturing filtered.

Specifications

• Antigen: Mouse IgG1 kappa
• Classification: Control
• Concentration: 5μl/Test
• Formulation: PBS with 0.1% gelatin, 0.2% BSA and 0.09% sodium azide; pH 7.2
• Gene Alias: IgG1, IgG1k
• Isotype: IgG1, kappa
• Quantity: 25 Tests
• Storage Requirements: 4° C, store in dark, DO NOT FREEZE!
• Monoclonal or Polyclonal: Monoclonal

• Applications: Control, Flow Cytometry, Immunocytochemistry, Immunofluorescence, Immunohistochemistry
• Clone: P3.6.2.8.1
• Conjugate: Alexa Fluor 488
• Format: Liquid
• Host Species: Murine
• Purification Method: Affinity chromatography
• Regulatory Status: RUO
• Type: Isotype Control
• Target Species: Not Applicable

For Research Use Only.