Mouse IgG1 kappa, eFluor 615, clone: P18.104.22.168.1, Isotype Control, eBioscience
Mouse Isotype Control
Manufacturer: Invitrogen 42471480
The isotype of a primary antibody and the application it is being used in can result in background staining. Primary antibody background noise can be caused by binding to Fc receptors on target cells; by non-specific interactions with cellular proteins, carbohydrates, and lipids; or by cell autofluorescence. Isotype control antibodies can act as negative controls to help differentiate non-specific background signal from specific antibody signal because they have no relevant specificity to a target antigen. While isotype controls are most commonly used in flow cytometry, they are useful in other applications such as chromatin immunoprecipitation (ChIP), immunohistochemistry, and gel shifts. Isotype controls should match with the primary antibody species and isotype so that the level of specific staining by the primary antibody may be accurately determined. If using directly labeled primary antibodies, the isotype control works best if conjugated with the same label as the test antibody.Description: The monoclonal mouse IgG1 kappa immunoglobulin is useful as an isotype control.Applications Reported: Mouse IgG1 K Isotype Control eFluor 615 has been reported for use in immunocytochemical (ICC) and immunohistochemical (IHC-P) staining.Applications Tested: This Mouse IgG1, K Isotype Control eFluor 615 has been tested by immunocytochemistry on fixed and permeabilized MDCK cells and by immunohistochemistry (IHC-P) on formalin-fixed paraffin embedded human brain tissue. Use isotype control at the same concentration as experimental antibody. Filter Recommendation: When using this eFluor 615 antibody conjugate, we recommend a filter that will capture the 615 emission wavelength. A standard Alexa Fluor 594 filter is acceptable.Excitation: 595 nm; Emission: 615 nm.Filtration: 0.2µm post-manufacturing filtered.
|Mouse IgG1 kappa|
|PBS with 0.1% gelatin and 0.09% sodium azide; pH 7.2|
|4° C, store in dark, DO NOT FREEZE!|
|Control, Immunocytochemistry, Immunofluorescence, Immunohistochemistry|
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