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Rat IgG1 kappa Isotype Control (eBRG1), Functional Grade, eBioscience™, Invitrogen™
Rat Isotype Control
Supplier: Invitrogen 16430181
Description
Description: The monoclonal rat IgG1, kappa is useful as an isotype control immunoglobulin. Applications Reported: This rat IgG1 isotype control has been reported for use in flow cytometric analysis. Applications Tested: Rat IgG1 Isotype Control has been tested by flow cytometric analysis of mouse splenocyte suspensions. It should be used at the same concentration as the experimental antibody. Storage and handling: Use in a sterile environment. Filtration: 0.2 μm post-manufacturing filtered. Purity: Greater than 90%, as determined by SDS-PAGE. Endotoxin Level: Less than 0.001 ng/μg antibody, as determined by LAL assay. Aggregation: Less than 10%, as determined by HPLC.
Rat IgG1 is a subclass of immunoglobulin G (IgG) antibodies that are commonly used in research and diagnostics. These antibodies are produced by rats in response to an antigen and can be purified from serum or hybridoma cell culture supernatants. Rat IgG1 is often used as a negative control in experiments, as it does not bind to many mammalian antigens. It is also used in immunohistochemistry, flow cytometry, and western blotting to detect specific antigens. Gene targeting is a powerful tool used to manipulate the rat genome and generate animal models for studying human diseases. With gene targeting, specific genes can be knocked out, inserted, or modified to investigate their role in disease pathogenesis.
Specifications
| Rat IgG1 kappa | |
| eBRG1 | |
| Functional Grade | |
| Immunoglobulin G; Immunoglobulin G1; ImmunoglobulinG; ImmunoglobulinG1 | |
| Affinity chromatography | |
| RUO | |
| Not Applicable | |
| Liquid |
| Control, Flow Cytometry, Functional Assay | |
| 1 mg/mL | |
| PBS with no preservative; pH 7.2 | |
| Rat | |
| 50 μg | |
| Isotype Control (Primary) | |
| 4° C | |
| IgG1 κ |
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