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Rat IgG1 kappa, eFluor 450, clone: eBRG1, Isotype Control, eBioscience™ Non-distribution product as customer accommodation.

Rat Isotype Control

Manufacturer:  Invitrogen 48430182

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Catalog No. 50-112-4162


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Description

Description

The isotype of a primary antibody and the application it is being used in can result in background staining. Primary antibody background noise can be caused by binding to Fc receptors on target cells; by non-specific interactions with cellular proteins, carbohydrates, and lipids; or by cell autofluorescence. Isotype control antibodies can act as negative controls to help differentiate non-specific background signal from specific antibody signal because they have no relevant specificity to a target antigen. While isotype controls are most commonly used in flow cytometry, they are useful in other applications such as chromatin immunoprecipitation (ChIP), immunohistochemistry, and gel shifts. Isotype controls should match with the primary antibody species and isotype so that the level of specific staining by the primary antibody may be accurately determined. If using directly labeled primary antibodies, the isotype control works best if conjugated with the same label as the test antibody.

Description: The monoclonal rat IgG1, kappa is useful as an isotype control immunoglobulin.Applications Reported: Rat IgG1 Isotype Control eFluor™ 450 has been reported for use in flow cytometric analysis.Applications Tested: eFluor™ 450 Rat IgG1 Isotype Control has been tested by flow cytometric analysis of mouse splenocytes. Use isotype control at the same concentration as experimental antibody. eFluor™ 450 is an alternative to Pacific Blue™. eFluor™ 450 emits at 445 nm and is excited with the Violet laser (405 nm). Please make sure that your instrument is capable of detecting this fluorochome.Excitation: 405 nm; Emission: 445 nm; Laser: Violet Laser.Filtration: 0.2µm post-manufacturing filtered.
Specifications

Specifications

Rat IgG1 kappa
Control
0.2mg/mL
PBS with 0.1% gelatin and 0.09% sodium azide; pH 7.2
IgG1, IgG1k
IgG1, kappa
100μg
Store at 2-8°C. Do not freeze. Light-sensitive material.
Monoclonal
Control, Flow Cytometry
eBRG1
eFluor 450
Liquid
Rat
Affinity chromatography
RUO
Isotype Control
Not Applicable
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