IgG1 Rat anti-Mouse, Brilliant Violet 605, Clone: X56, BD Optibuild™
Rat Monoclonal Antibody
Manufacturer: BD Biosciences 742477
The X56 monoclonal antibody specifically binds to mouse IgG1 of Igh-C[a] /and Igh-C[b] /haplotypes. It does not crossreact with other Ig isotypes.This antibody is conjugated to BD Horizon™ BV605 which is part of the BD Horizon Brilliant™ Violet family of dyes. With an Ex Max of 407nm and Em Max of 602nm, BD Horizon BV605 can be excited by a violet laser and detected with a standard 610/20nm filter set. BD Horizon BV605 is a tandem fluorochrome of BD Horizon BV421 and an acceptor dye with an Em max at 605nm. Due to the excitation of the acceptor dye by the green (532 nm) and yellow-green (561 nm) lasers, there will be significant spillover into the PE and BD Horizon PE-CF594 detectors off the green or yellow-green lasers. BD Horizon BV605 conjugates are very bright, often exhibiting brightness equivalent to PE conjugates and can be used as a third color off of the violet laser. BV605 is a tandem fluorochrome that combines BD Horizon BV421 and Cy™3.5. These conjugates are very bright, exhibiting similar brightness to equivalent PE conjugates. Due to the excitation of Cy3.5 by the green (532nm) and yellow-green (561nm) lasers, there will be significant spillover of the BD Horizon BV605 signal into the PE and BD Horizon PE-CF594 detectors off the green or yellow-green lasers.
|Aqueous buffered solution containing ≤0.09% sodium azide.|
|Igh-4, Ighg1, Immunoglobulin heavy constant gamma 1|
|Mouse IgG1, κ Antibody|
|The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with BD Horizon BV605 under optimal conditions that minimize unconjugated dye and antibody.|
|Brilliant Violet 605|
|Rat LOU, also known as Louvain, LOU/C, LOU/M IgG1, κ|
|Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.|
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