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Rat anti-Mouse IgG1 Secondary Antibody, HRP, eBioscience™, Invitrogen™

Rat Monoclonal Secondary Antibody

Supplier:  Invitrogen 18401580

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Catalog No. 50-112-3247


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Description

Description

Description: The monoclonal antibody M1-14D12 recognizes Mouse IgG1 antibodies and can be used as a second step reagent in flow cytometry and microscopy. The monoclonal does not recognize other mouse isotypes nor does it crossreact to rat IgG1 or any rat isotype antibodies. Applications Reported: This M1-14D12 antibody has been reported for use in western blotting, ELISA, immunohistochemical and immunocytochemical staining. Applications Tested: This M1-14D12 antibody has been tested by immunocytochemistry and immunohistochemistry after staining with a primary antibody of the mouse IgG1 isotype. This can be used at less than or equal to 20 μg/mL. For western blotting, this can be used at 1 μg/mL (1/500 dilution). It is recommended that the antibody be titrated for optimal performance in the assay of interest.

Mouse IgG1 Monoclonal antibody specifically detects Mouse IgG1 in Mouse samples. It is validated for ELISA, Immunocytochemistry, Immunohistochemistry (Paraffin), Western Blot.
TRUSTED_SUSTAINABILITY
Specifications

Specifications

Mouse IgG1
Monoclonal
0.5 mg/mL
PBS with no preservative; pH 7.2
Affinity chromatography
RUO
Mouse
Liquid
ELISA, Immunocytochemistry, Immunohistochemistry (Paraffin), Western Blot
M1-14D12
HRP
Rat
25 μg
Secondary
4° C
IgG
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