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Mouse IgG2a kappa, eFluor 450, clone: eBM2a, Isotype Control, eBioscience™ Non-distribution product as customer accommodation.

Mouse Isotype Control

Manufacturer:  Invitrogen 48472480

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Catalog No. 50-112-8790


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Description

Description

The isotype of a primary antibody and the application it is being used in can result in background staining. Primary antibody background noise can be caused by binding to Fc receptors on target cells; by non-specific interactions with cellular proteins, carbohydrates, and lipids; or by cell autofluorescence. Isotype control antibodies can act as negative controls to help differentiate non-specific background signal from specific antibody signal because they have no relevant specificity to a target antigen. While isotype controls are most commonly used in flow cytometry, they are useful in other applications such as chromatin immunoprecipitation (ChIP), immunohistochemistry, and gel shifts. Isotype controls should match with the primary antibody species and isotype so that the level of specific staining by the primary antibody may be accurately determined. If using directly labeled primary antibodies, the isotype control works best if conjugated with the same label as the test antibody.

Description: This is a monoclonal mouse IgG2a, kappa antibody. It is used as an isotype control for mouse IgG2a antibodies.Applications Reported: Mouse IgG2a K Isotype Control eFluor™ 450 has been reported for use in flow cytometric analysis.Applications Tested: eFluor™ 450 Mouse IgG2a, K Isotype Control has been tested by flow cytometric analysis of normal human peripheral blood cells. Use isotype control at the same concentration as experimental antibody.Excitation: 405 nm; Emission: 445 nm; Laser: Violet Laser.Filtration: 0.2µm post-manufacturing filtered.
Specifications

Specifications

Mouse IgG2a kappa
Control
0.2mg/mL
PBS with 0.1% gelatin and 0.09% sodium azide; pH 7.2
IgG2, IgG2a, IgG2ak
IgG2a, kappa
25μg
Store at 2-8°C. Do not freeze. Light-sensitive material.
Monoclonal
Control, Flow Cytometry
eBM2a
eFluor 450
Liquid
Murine
Affinity chromatography
RUO
Isotype Control
Not Applicable
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