Rat IgG2a kappa, Alexa Fluor 700, clone: eBR2a, Isotype Control, eBioscience
Rat Isotype Control
Manufacturer: Invitrogen 56432180
The isotype of a primary antibody and the application it is being used in can result in background staining. Primary antibody background noise can be caused by binding to Fc receptors on target cells; by non-specific interactions with cellular proteins, carbohydrates, and lipids; or by cell autofluorescence. Isotype control antibodies can act as negative controls to help differentiate non-specific background signal from specific antibody signal because they have no relevant specificity to a target antigen. While isotype controls are most commonly used in flow cytometry, they are useful in other applications such as chromatin immunoprecipitation (ChIP), immunohistochemistry, and gel shifts. Isotype controls should match with the primary antibody species and isotype so that the level of specific staining by the primary antibody may be accurately determined. If using directly labeled primary antibodies, the isotype control works best if conjugated with the same label as the test antibody.Description: The monoclonal rat IgG2a, kappa is useful as an isotype control immunoglobulin.Applications Reported: This eBR2a antibody has been reported for use in flow cytometric analysis.Applications Tested: This eBR2a antibody has been tested by flow cytometric analysis of mouse splenocytes and normal human peripheral blood cells. It should be used at the same concentration as the experimental antibody.Alexa Fluor 700 emits at 723 nm and can be excited with the red laser (633 nm). Most instruments will require a 685 LP mirror and 710/20 filter. Please make sure that your instrument is capable of detecting this fluorochrome.Excitation: 633-647 nm; Emission: 723 nm; Laser: Red Laser.Filtration: 0.2µm post-manufacturing filtered.
|Rat IgG2a kappa|
|PBS with 0.1% gelatin and 0.09% sodium azide; pH 7.2|
|IgG2, IgG2a, IgG2k|
|Store at 2-8°C. Do not freeze. Light-sensitive material.|
|Control, Flow Cytometry|
|Alexa Fluor 700|
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