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Rat IgG2a kappa Isotype Control (eBR2a), APC-eFluor™ 780, eBioscience™, Invitrogen™
Rat Isotype Control
Supplier: Invitrogen 47432180
Description
Description: The monoclonal rat IgG2a, kappa is useful as an isotype control immunoglobulin. Applications Reported: APC-eFluor™ 780 Rat IgG2a Isotype Control (APC-Alexa Fluor™ 750 replacement) has been reported for use in flow cytometric analysis. Applications Tested: Rat IgG2a K Isotype Control APC-eFluor™ 780 has been tested by flow cytometric analysis of mouse splenocytes. Use isotype control at the same concentration as experimental antibody. APC-eFluor 780 emits at 780 nm and is excited with the Red laser (633 nm). Please make sure that your instrument is capable of detecting this fluorochome. Light sensitivity: This tandem is sensitive to photo-induced oxidation. Please protect this vial and stained samples from light. Fixation: Samples can be stored in IC Fixation Buffer (cat. 00-8222) (100 μL cell sample + 100 μL IC Fixation Buffer) or 1-step Fix/Lyse Solution (cat. 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically. Excitation: 633-647 nm; Emission: 780 nm; Laser: Red Laser. Filtration: 0.2 μm post-manufacturing filtered.
Rat IgG2a kappa Control antibody specifically detects Rat IgG2a kappa in Not Applicable samples. It is validated for Control, Flow Cytometry.
Specifications
| Rat IgG2a kappa | |
| eBR2a | |
| APC-eFluor 780 | |
| IgG2; Immunoglobulin G; Immunoglobulin G2; ImmunoglobulinG; ImmunoglobulinG2 | |
| Affinity chromatography | |
| RUO | |
| Not Applicable | |
| Liquid |
| Control, Flow Cytometry | |
| 0.2 mg/mL | |
| PBS with 0.09% sodium azide; pH 7.2 | |
| Rat | |
| 25 μg | |
| Isotype Control (Primary) | |
| 4° C, store in dark, DO NOT FREEZE! | |
| IgG2a κ |
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