Rat IgG2a kappa Isotype Control (eBR2a), eBioscience™, Invitrogen™

Rat Isotype Control

Catalog Number	Mfr. No.	Quantity
50-130-37	Invitrogen 14432181	50 μg
50-112-9472	Invitrogen 14432182	100 μg
50-112-9680	Invitrogen 14432185	500 μg

Description

Description: The monoclonal rat IgG2a, kappa is useful as an isotype control immunoglobulin. Applications Reported: This rat IgG2a isotype control has been reported for use in immunocytochemistry, immunohistochemistry, flow cytometric analysis, and immunoglobulin ELISA. Applications Tested: This rat IgG2a isotype control has been used by flow cytometric analysis of mouse splenocyte suspension and should be used at the same concentration as the experimental antibody. Purity: Greater than 90%, as determined by SDS-PAGE. Aggregation: Less than 10%, as determined by HPLC. Filtration: 0.2 μm post-manufacturing filtered.

Rat IgG2a is an immunoglobulin subclass that is commonly used in research and diagnostic applications. It is produced by the rat immune system and is known for its ability to activate complement and mediate antibody-dependent cell-mediated cytotoxicity (ADCC). Rat IgG2a has a molecular weight of approximately 160 kDa and is composed of two heavy chains and two light chains. It is often used as a control or reference antibody in experiments involving rat models, and is also commonly used in flow cytometry and immunohistochemistry applications.

Specifications

• Antigen: Rat IgG2a kappa
• Concentration: 0.5 mg/mL
• Conjugate: Unconjugated
• Host Species: Rat
• Target Species: Not Applicable
• Gene Alias: IgG2; Immunoglobulin G; Immunoglobulin G2; ImmunoglobulinG; ImmunoglobulinG2
• Primary or Secondary: Isotype Control (Primary)
• Content And Storage: 4° C

• Clone: eBR2a
• Applications: Control, Flow Cytometry, Immunocytochemistry, Immunohistochemistry, Immunohistochemistry
• Form: Liquid
• Regulatory Status: RUO
• Formulation: PBS with 0.09% sodium azide; pH 7.2
• Isotype: IgG2a κ
• Purification Method: Affinity chromatography

For Research Use Only.