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Rat IgG2a kappa, eFluor 570, clone: eBR2a, Isotype Control, eBioscience™ Non-distribution product as customer accommodation.

Rat Isotype Control

Manufacturer:  Invitrogen 41432182

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Catalog No. 50-112-3614


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Description

Description

The isotype of a primary antibody and the application it is being used in can result in background staining. Primary antibody background noise can be caused by binding to Fc receptors on target cells; by non-specific interactions with cellular proteins, carbohydrates, and lipids; or by cell autofluorescence. Isotype control antibodies can act as negative controls to help differentiate non-specific background signal from specific antibody signal because they have no relevant specificity to a target antigen. While isotype controls are most commonly used in flow cytometry, they are useful in other applications such as chromatin immunoprecipitation (ChIP), immunohistochemistry, and gel shifts. Isotype controls should match with the primary antibody species and isotype so that the level of specific staining by the primary antibody may be accurately determined. If using directly labeled primary antibodies, the isotype control works best if conjugated with the same label as the test antibody.

Description: The monoclonal rat IgG2a is useful as an isotype control immunoglobulin.Applications Reported: This eBR2a antibody has been reported for use in immunohistochemical and immunocytochemical staining.Applications Tested: This Rat IgG2a Isotype Control eFluor™ 570 has been tested by immunocytochemistry on fixed and permeabilized C2C12 cells and by immunohistochemistry (IHC-P) on formalin-fixed paraffin embedded human tissue. Use isotype control at the same concentration as experimental antibody. Filter Recommendation: When using this eFluor™ 570 antibody conjugate, we recommend a filter that will capture the 570 emission wavelength (for example, Excitation 545/25, 565LP, Emission 605/70). A standard Alexa Fluor™ 555 or TRITC filter is acceptable.Excitation: 555 nm; Emission: 570 nm
Specifications

Specifications

Rat IgG2a kappa
Control
0.2mg/mL
PBS with 0.1% gelatin and 0.09% sodium azide; pH 7.2
IgG2, IgG2a, IgG2k
IgG2a, kappa
100μg
Store at 2-8°C. Do not freeze. Light-sensitive material.
Monoclonal
Control, Immunocytochemistry, Immunofluorescence, Immunohistochemistry
eBR2a
eFluor 570
Liquid
Rat
Affinity chromatography
RUO
Isotype Control
Not Applicable
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