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IgG2a Mouse anti-Rat, eFluor® 660, eBioscience™ Non-distribution product as customer accommodation.

Mouse Monoclonal Secondary Antibody

$182.00

Specifications

Antigen Rat IgG2a
Clone r2a-21B2
Target Species Rat
Conjugate eFluor™ 660
Isotype IgG1, kappa
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Products 1
Catalog Number Mfr. No. Quantity Price Quantity & Availability  
Catalog Number Mfr. No. Quantity Price Quantity & Availability  
50-112-4373 Non-distribution product as customer accommodation. View Documents Invitrogen
50481782
100 μg Each for $182.00
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Description

Description

Description: The monoclonal antibody r2a-21B2 recognizes Rat IgG2a antibodies and can be used as a second step reagent in flow cytometry and microscopy. The monoclonal does not recognize other rat isotypes nor does it crossreact to mouse IgG2a or any mouse isotype antibodies. Applications Reported: This r2a-21B2 antibody has been reported for use in flow cytometric analysis. Applications Tested: This r2a-21B2 antibody has been tested by flow cytometric analysis of mouse splenocytes and normal human peripheral blood cells stained with a rat IgG2a primary antibody. This can be used at less than or equal to 0.25 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. eFluor® 660 is a replacement for Alexa Fluor® 647. eFluor® 660 emits at 659 nm and is excited with the red laser (633 nm). Please make sure that your instrument is capable of detecting this fluorochome. Excitation: 633-647 nm; Emission: 668 nm; Laser: Red Laser. Filtration: 0.2 µm post-manufacturing filtered.

Anti-Rat secondary antibodies are affinity-purified antibodies with well-characterized specificity for rat immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.
Specifications

Specifications

Rat IgG2a
Rat
IgG1, kappa
Monoclonal
Liquid
Secondary Antibody
4° C, store in dark, DO NOT FREEZE!
r2a-21B2
eFluor™ 660
Flow Cytometry
Secondary
Monoclonal
PBS with 0.1% gelatin and 0.09% sodium azide; pH 7.2
RUO

For Research Use Only.

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