Promotional price valid on web orders only. Your contract pricing may differ. Interested in signing up for a dedicated account number?
Learn More

IgG2a Mouse anti-Rat, FITC, eBioscience™ Non-distribution product as customer accommodation.

Mouse Monoclonal Secondary Antibody

Manufacturer:  Invitrogen 11481782

Catalog No. 50-112-2130


Add to cart

Description

Description

Description: The monoclonal antibody r2a-21B2 recognizes Rat IgG2a antibodies and can be used as a second step reagent in flow cytometry and microscopy. The monoclonal does not recognize other rat isotypes nor does it crossreact to mouse IgG2a or any mouse isotype antibodies. Applications Reported: This r2a-21B2 antibody has been reported for use in flow cytometric analysis, immunohistochemical staining of formalin-fixed paraffin embedded tissue sections, and immunocytochemistry. Applications Tested: This r2a-21B2 antibody has been tested by flow cytometric analysis and immunohistochemstry using rat IgG2a primary antibody. This can be used at less than or equal to 0.5 µg per test for flow cytometry or 10 µg/mL for IHC. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Excitation: 488 nm; Emission: 520 nm; Laser: Blue Laser. Filtration: 0.2 µm post-manufacturing filtered.

Anti-Rat secondary antibodies are affinity-purified antibodies with well-characterized specificity for rat immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.
Specifications

Specifications

Rat IgG2a
Monoclonal
0.5 mg/mL
PBS with 0.1% gelatin and 0.09% sodium azide; pH 7.2
Mouse
Affinity chromatography
RUO
Secondary Antibody
Monoclonal
Flow Cytometry, Immunocytochemistry, Immunofluorescence, Immunohistochemistry (Paraffin)
r2a-21B2
FITC
Liquid
IgG1, kappa
100 μg
4° C, store in dark, DO NOT FREEZE!
Secondary
Rat
Documents
Provide Content Correction

The Fisher Scientific Encompass Program offers items which are not part of our distribution portfolio. These products typically do not have pictures or detailed descriptions. However, we are committed to improving your shopping experience. Please use the form below to provide feedback related to the content on this product.

Product Title

By clicking Submit, you acknowledge that you may be contacted by Fisher Scientific in regards to the feedback you have provided in this form. We will not share your information for any other purposes. All contact information provided shall also be maintained in accordance with our Privacy Policy.

Cancel Submit

For Research Use Only.