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IgG2a, κ Rat, Brilliant Violet 750, Clone: R35-95  , Isotype Control, BD

Rat Monoclonal Antibody

Manufacturer:  BD Biosciences 566362

Catalog No. BDB566362


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Description

Description

The R35-95 hybridoma was generated by hybridization of Y3 myeloma cells with spleen cells from LOU rats immunized with mouse immunoglobulins. The R35-95 hybridoma produces rat IgG2a, κ immunoglobulin that has no measurable reactivity with mouse immunoglobulins. The R35-95 immunoglobulin was selected as an isotype control following screening for low background binding on a variety of mouse and human tissues.

The antibody was conjugated to BD Horizon BV750 which is part of the BD Horizon Brilliant™ Violet family of dyes. This dye is a tandem fluorochrome of BD Horizon BV421 with an Ex Max of 405nm and an acceptor dye with an Em Max at 750nm. BD Horizon Brilliant BV750 can be excited by the violet laser (405 nm) and detected with a 750/30 nm filter with a 740 nm long pass. Due to spectral differences between labeled cells and beads, using BD™ CompBeads can result in incorrect spillover values when used with BD Horizon BV750 reagents. Therefore, the use of BD CompBeads or BD CompBeads Plus to determine spillover values for these reagents is not recommended. BV750
Specifications

Specifications

Rat IgG2a, κ Isotype Control
0.2mg/mL
Aqueous buffered solution containing ≤0.09% sodium azide.
Rat
Rat LOU, also known as Louvain, LOU/C, LOU/M IgG2a, κ
RUO
Primary
R35-95
Brilliant Violet 750
Rat IgG2a kappa Isotype Control
Mouse Pooled Immunoglobulin
50μg
Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
Monoclonal
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