Rat IgG2b kappa, PE-eFluor 610, clone: eB149/10H5, Isotype Control, eBioscience
Rat Isotype Control
Manufacturer: Invitrogen 61403180
The isotype of a primary antibody and the application it is being used in can result in background staining. Primary antibody background noise can be caused by binding to Fc receptors on target cells; by non-specific interactions with cellular proteins, carbohydrates, and lipids; or by cell autofluorescence. Isotype control antibodies can act as negative controls to help differentiate non-specific background signal from specific antibody signal because they have no relevant specificity to a target antigen. While isotype controls are most commonly used in flow cytometry, they are useful in other applications such as chromatin immunoprecipitation (ChIP), immunohistochemistry, and gel shifts. Isotype controls should match with the primary antibody species and isotype so that the level of specific staining by the primary antibody may be accurately determined. If using directly labeled primary antibodies, the isotype control works best if conjugated with the same label as the test antibody.Description: The rat IgG2b monoclonal antibody is useful as an isotype control immunoglobulin.Applications Reported: Rat IgG2b K Isotype Control PE-eFluor 610 has been reported for use in flow cytometric analysis.Applications Tested: This Rat IgG2b K Isotype Control PE-eFluor 610 has been tested by flow cytometric analysis of normal human peripheral blood cells and mouse spleen cells. Use isotype control at thesame concentration as experimental antibody.PE-eFluor 610 can be excited with laser lines from 488-561 nm and emits at 607 nm. We recommend using a 610/20 band pass filter (equivalent to PE-Texas Red). Please make sure that your instrument is capable of detecting this fluorochome.Light sensitivity: This tandem dye is sensitive to photo-induced oxidation. Please protect this vial and stained samples from light.Fixation: Samples can be stored in IC Fixation Buffer (cat. 00-8222) (100 µL of cell sample + 100 µL of IC Fixation Buffer) or 1-step Fix/Lyse Solution (cat. 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.Excitation: 488-561 nm; Emission: 607 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.Filtration: 0.2µm post-manufacturing filtered.
|Rat IgG2b kappa|
|PBS with 0.09% sodium azide; pH 7.2|
|IgG2, IgG2b, IgG2bk|
|Store at 2-8°C. Do not freeze. Light-sensitive material. This tandem dye is sensitive to photo-induced oxidation. Protect this vial from light during storage|
|Control, Flow Cytometry|
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