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Rat IgG2b kappa, PerCP-Cyanine5.5, clone: eB149/10H5, Isotype Control, eBioscience™ Non-distribution product as customer accommodation.

Rat Isotype Control

Manufacturer:  Invitrogen 45403180

Catalog No. 50-112-9676


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Description

Description

The isotype of a primary antibody and the application it is being used in can result in background staining. Primary antibody background noise can be caused by binding to Fc receptors on target cells; by non-specific interactions with cellular proteins, carbohydrates, and lipids; or by cell autofluorescence. Isotype control antibodies can act as negative controls to help differentiate non-specific background signal from specific antibody signal because they have no relevant specificity to a target antigen. While isotype controls are most commonly used in flow cytometry, they are useful in other applications such as chromatin immunoprecipitation (ChIP), immunohistochemistry, and gel shifts. Isotype controls should match with the primary antibody species and isotype so that the level of specific staining by the primary antibody may be accurately determined. If using directly labeled primary antibodies, the isotype control works best if conjugated with the same label as the test antibody.

Description: The rat IgG2b monoclonal antibody is useful as an isotype control immunoglobulin.Applications Reported: PerCP-Cy5.5 Rat IgG2b Isotype Control has been reported for use in flow cytometric analysis.Applications Tested: Rat IgG2b Isotype Control has been tested by flow cytometric analysis of mouse splenocyte suspensions. It should be used at the same concentration as the experimental antibody.Excitation: 488 nm; Emission: 695 nm; Laser: Blue Laser.Filtration: 0.2µm post-manufacturing filtered.
Specifications

Specifications

Rat IgG2b kappa
Control
0.2mg/mL
PBS with 0.1% gelatin and 0.09% sodium azide; pH 7.2
IgG2, IgG2b, IgG2bk
IgG2b, kappa
25μg
Store at 2-8°C. Do not freeze. Light-sensitive material.
Monoclonal
Control, Flow Cytometry
eB149/10H5
PerCP-Cyanine5.5
Liquid
Rat
Affinity chromatography
RUO
Isotype Control
Not Applicable
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