Rat IgG2b kappa Isotype Control (eB149/10H5), PerCP-eFluor™ 710, eBioscience™, Invitrogen™

Rat Isotype Control

Supplier:  Invitrogen 46403182

Catalog No. 50-160-40

Description

Description: The rat IgG2b, kappa monoclonal antibody is useful as an isotype control immunoglobulin. Applications Reported: PerCP-eFluor® 710 Rat IgG2b Isotype Control has been reported for use in flow cytometric analysis. Applications Tested: PerCP-eFluor® 710 Rat IgG2b Isotype Control has been tested by flow cytometric analysis of normal human peripheral blood cells and mouse spleen cells.Use at the same concentration as experimental antibody. PerCP-eFluor® 710 can be used in place of PE-Cy5, PE-Cy5.5 or PerCP-Cy5.5. PerCP-eFluor® 710 emits at 710 nm and is excited with the blue laser (488 nm). Please make sure that your instrument is capable of detecting this fluorochrome. For a filter configuration, we recommend using the 685 LP dichroic mirror and 710/40 band pass filter, however the 695/40 band pass filter is an acceptable alternative. Our testing indicates that PerCP-eFluor® 710 conjugated antibodies are stable when stained samples are exposed to freshly prepared 2% formaldehyde overnight at 4°C, but please evaluate for alternative fixation protocols. Excitation: 488 nm; Emission: 710 nm; Laser: Blue Laser. Filtration: 0.2 μm post-manufacturing filtered.

Rat IgG2b kappa Control antibody specifically detects Rat IgG2b kappa in Not Applicable samples. It is validated for Control, Flow Cytometry.

Specifications

• Antigen: Rat IgG2b kappa
• Clone: eB149/10H5
• Conjugate: PerCP-eFluor 710
• Gene Alias: IgG2; Immunoglobulin G; Immunoglobulin G2; ImmunoglobulinG; ImmunoglobulinG2
• Purification Method: Affinity chromatography
• Regulatory Status: RUO
• Target Species: Not Applicable
• Form: Liquid

• Applications: Control, Flow Cytometry
• Concentration: 0.2 mg/mL
• Formulation: PBS with 0.09% sodium azide; pH 7.2
• Host Species: Rat
• Quantity: 100 μg
• Primary or Secondary: Isotype Control (Primary)
• Content And Storage: 4° C, store in dark, DO NOT FREEZE!
• Isotype: IgG2b κ

For Research Use Only.