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IgG2b Mouse anti-Rat, eFluor® 570, eBioscience™ Non-distribution product as customer accommodation.

Mouse Monoclonal Secondary Antibody

Manufacturer:  Invitrogen 41481580

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Catalog No. 50-112-3624


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Description

Description

Description: The monoclonal antibody R2B-7C3 recognizes Rat IgG2b antibodies and can be used as a second step reagent in flow cytometry and microscopy. The monoclonal does not recognize other rat isotypes nor does it crossreact to mouse IgG2b or any mouse isotype antibodies. Applications Reported: This R2B-7C3 antibody has been reported for use in immunohistochemical staining of frozen tissue sections, immunohistochemical staining of formalin-fixed paraffin embedded tissue sections, and immunocytochemistry. Applications Tested: This R2B-7C3 antibody has been tested by immunohistochemistry on formalin-fixed paraffin embedded sections that have been stained first with a rat IgG2b anti-human antibody. This can be used at less than or equal to 5 µg/mL. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Filter Recommendation: When using this eFluor® 570 antibody conjugate, we recommend a filter that will capture the 570 emission wavelength (for example, Excitation 545/25, 565LP, Emission 605/70). A standard Alexa Fluor® 555 or TRITC filter is acceptable. Excitation: 555 nm; Emission: 570 nm

Secreted immunoglobulins (Ig) have two separable functions: one is to bind specifically to pathogenic molecules, the other is to recruit cells and molecules to destroy the opsonized pathogen.
Specifications

Specifications

Rat IgG2b
Monoclonal
0.2 mg/mL
PBS with 0.1% gelatin and 0.09% sodium azide; pH 7.2
Mouse
Affinity chromatography
RUO
Secondary Antibody
Monoclonal
Immunocytochemistry, Immunofluorescence, Immunohistochemistry (Frozen), Immunohistochemistry (Paraffin)
R2B-7C3
eFluor™ 570
Liquid
IgG2b, kappa
25 μg
4° C, store in dark, DO NOT FREEZE!
Secondary
Rat
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