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IgM[b] Mouse anti-Mouse, Brilliant Violet 510, Clone: AF6-78, BD Optibuild™

Mouse Monoclonal Antibody

Manufacturer:  BD Biosciences 742344

Catalog No. BDB742344


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Description

Description

The AF6-78 antibody reacts specifically with mouse IgM of Igh-C[b] /and related haplotypes (e.g., C57BL/6, C57BL/10, SJL, AKR, NZB). It does not react with IgM of Igh-C[a] /or related haplotypes (e.g., BALB/c, C58, CBA, C3H/Bi, C3H/He, DBA/1, DBA/2, PL, RIII). Cross-reaction with IgM of Igh-C[e] /haplotype (e.g., A/J) has been observed. AF6-78 antibody does not react with free μ heavy chain in vitro or in the cytoplasm of pre-B lymphocytes, which lack Ig light chain. It has not been shown to stimulate B-cell proliferation.

The antibody was conjugated to BD Horizon™ BV510 which is part of the BD Horizon Brilliant™ Violet family of dyes. With an Ex Max of 405nm and Em Max at 510nm, BD Horizon BV510 can be excited by the violet laser and detected in the BD Horizon V500 (525/50nm) filter set. BD Horizon BV510 conjugates are useful for the detection of dim markers off the violet laser. BV510 is a polymer-based dye that is brighter than BD Horizon V500. Due to similar excitation and emission properties, BD Horizon BV510 and BD Horizon V500 cannot be used simultaneously.
Specifications

Specifications

IgM[b]
0.2mg/mL
Aqueous buffered solution containing ≤0.09% sodium azide.
Igh-6b
C57BL/10 mouse splenocytes
The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with BD Horizon BV510 under optimal conditions that minimize unconjugated dye and antibody.
RUO
Primary
Murine
AF6-78
Brilliant Violet 510
Affinity Purified
Murine
Mouse BALB/c IgG1, κ
50μg
Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
Monoclonal
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