IgM[b] Mouse anti-Mouse, Brilliant Violet 605, Clone: AF6-78, BD Optibuild™
Mouse Monoclonal Antibody
Manufacturer: BD Biosciences 742345
The AF6-78 antibody reacts specifically with mouse IgM of Igh-C[b] /and related haplotypes (e.g., C57BL/6, C57BL/10, SJL, AKR, NZB). It does not react with IgM of Igh-C[a] /or related haplotypes (e.g., BALB/c, C58, CBA, C3H/Bi, C3H/He, DBA/1, DBA/2, PL, RIII). Cross-reaction with IgM of Igh-C[e] /haplotype (e.g., A/J) has been observed. AF6-78 antibody does not react with free μ heavy chain in vitro or in the cytoplasm of pre-B lymphocytes, which lack Ig light chain. It has not been shown to stimulate B-cell proliferation.This antibody is conjugated to BD Horizon™ BV605 which is part of the BD Horizon Brilliant™ Violet family of dyes. With an Ex Max of 407nm and Em Max of 602nm, BD Horizon BV605 can be excited by a violet laser and detected with a standard 610/20nm filter set. BD Horizon BV605 is a tandem fluorochrome of BD Horizon BV421 and an acceptor dye with an Em max at 605nm. Due to the excitation of the acceptor dye by the green (532 nm) and yellow-green (561 nm) lasers, there will be significant spillover into the PE and BD Horizon PE-CF594 detectors off the green or yellow-green lasers. BD Horizon BV605 conjugates are very bright, often exhibiting brightness equivalent to PE conjugates and can be used as a third color off of the violet laser. BV605 is a tandem fluorochrome that combines BD Horizon BV421 and Cy™3.5. These conjugates are very bright, exhibiting similar brightness to equivalent PE conjugates. Due to the excitation of Cy3.5 by the green (532nm) and yellow-green (561nm) lasers, there will be significant spillover of the BD Horizon BV605 signal into the PE and BD Horizon PE-CF594 detectors off the green or yellow-green lasers.
|Aqueous buffered solution containing ≤0.09% sodium azide.|
|C57BL/10 mouse splenocytes|
|The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with BD Horizon BV605 under optimal conditions that minimize unconjugated dye and antibody.|
|Brilliant Violet 605|
|Mouse BALB/c IgG1, κ|
|Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.|
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