Invitrogen™ IgM Human Uncoated ELISA Kit

For quantitative detection of human IgM.

Supplier:  Invitrogen™ 885062088

Catalog No. 50-112-8719

Includes: Capture Antibody Concentrate

Description

The Human Immunoglobulin M (IgM) Uncoated ELISA Kit contains pre-matched antibody pairs and reagents for performing quantitative enzyme linked immunosorbent assays (ELISA) to detect and quantify protein levels of human IgM. Wash Buffer and Stop Solution are needed to complete the ELISA reaction and are sold separately. Principle of the method ELISAs are designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody is coated to the bottom of the wells of a microplate, which is an overnight process. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. A sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.

IgM is expressed intracellularly, during early stages of B lymphopoiesis, and then on the surface of more mature B cells in the bone marrow and peripheral B cells.

Specifications

• Assay Range: 15.6-1,000 ng/mL
• Conjugate: HRP
• Sample Type: Plasma, Serum, Supernatant
• For Use With (Application): ELISA
• Kit Contents: Capture Antibody: Pre-titrated; purified antibody, Detection Antibody: Pre-titrated; HRP-conjugated antibody, Standard: Recombinant protein for generating standard curve and calibrating samples, 10X Coating Buffer: Buffer for plating the Capture Antibody, 5X ELISA/ELISPOT Diluent: Buffer for blocking and diluting the Detection Antibody and Enzyme, Substrate: 1X TMB Solution, Certificate of Analysis: Lot-specific instructions for dilution of antibodies and standards, 96 Well Plate: Corning Costar 9018 (included with product Cat. Nos. ending in suffixes -22; -76; -86)
• Quantity: 10 x 96 Tests
• Sample Volume: Plasma, 10 μL; Serum, 10 μL; Supernatant, 10 μL
• Synonym: Immunoglobulin M
• Test Time: 1 hr. 45 min.

• Assay Sensitivity: 15.6 ng/mL
• Product Type: ELISA Kit
• Target Species: Human
• For Use With (Equipment): Colorimetric Microplate Reader
• Label or Dye: HRP
• Regulatory Status: RUO
• Storage Requirements: 2°C to 8°C
• Target: Human
• Total Assay Time: 24 hr. 30 min.

Safety and Handling

WARNING: Reproductive Harm - www.P65Warnings.ca.gov

For Research Use Only