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IgM Monoclonal Antibody (SA-DA4), PerCP-eFluor™ 710, eBioscience™, Invitrogen™

Mouse Monoclonal Antibody

$429.00

Specifications

Antigen IgM
Clone SA-DA4
Concentration 5 μL/Test
Applications Flow Cytometry
Classification Monoclonal
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Products 1
Catalog Number Mfr. No. Quantity Price Quantity & Availability  
Catalog Number Mfr. No. Quantity Price Quantity & Availability  
46-999-842
Encompass_Preferred
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Invitrogen
46999842
100 Tests
Each of 1 for $429.00
Add to Cart
 
Description

Description

Description: The SA-DA4 monoclonal antibody reacts with the mu heavy chain of human IgM. It does not react with other classes of human immunoglobulin including IgD, IgG, or IgA. IgM is expressed intracellularly, during early stages of B lymphopoiesis, and then on the surface of mature B cells. Applications Reported: This SA-DA4 antibody has been reported for use in flow cytometric analysis. Applications Tested: This SA-DA4 antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 μL (0.06 μg) per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. PerCP-eFluor™ 710 emits at 710 nm and is excited with the blue laser (488 nm); it can be used in place of PerCP-Cyanine5.5. We recommend using a 710/50 bandpass filter, however, the 695/40 bandpass filter is an acceptable alternative. Please make sure that your instrument is capable of detecting this fluorochrome. Fixation: Samples can be stored in IC Fixation Buffer (cat. 00-8222) (100 μL cell sample + 100 μL IC Fixation Buffer) or 1-step Fix/Lyse Solution (cat. 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation.

IgM Monoclonal antibody specifically detects IgM in Human samples. It is validated for Flow Cytometry.
Specifications

Specifications

IgM
5 μL/Test
Monoclonal
Liquid
RUO
P01871
IGHM
Primary
4° C, store in dark, DO NOT FREEZE!
IGHM
SA-DA4
Flow Cytometry
PerCP-eFluor 710
Mouse
Human
3507
IgG1 κ
Affinity chromatography
Antibody
SDS
Documents

Documents

Product Certifications

For Research Use Only.

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