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Mouse IgM, eFluor 660, clone: 11E10, Isotype Control, eBioscience™ Non-distribution product as customer accommodation.

Mouse Isotype Control

$118.00 - $246.00

Specifications

Antigen Mouse IgM
Clone 11E10
Target Species Not Applicable
Conjugate eFluor 660
Isotype IgM
View More Specs

Products 2
Catalog Number Mfr. No. Quantity Price Quantity & Availability  
Catalog Number Mfr. No. Quantity Price Quantity & Availability  
50-164-51 Non-distribution product as customer accommodation. View Documents Invitrogen
50475280
25μg Each for $118.00
Add to cart
 
50-164-52 Non-distribution product as customer accommodation. View Documents Invitrogen
50475282
100μg Each for $246.00
Add to cart
 
Description

Description

The isotype of a primary antibody and the application it is being used in can result in background staining. Primary antibody background noise can be caused by binding to Fc receptors on target cells; by non-specific interactions with cellular proteins, carbohydrates, and lipids; or by cell autofluorescence. Isotype control antibodies can act as negative controls to help differentiate non-specific background signal from specific antibody signal because they have no relevant specificity to a target antigen. While isotype controls are most commonly used in flow cytometry, they are useful in other applications such as chromatin immunoprecipitation (ChIP), immunohistochemistry, and gel shifts. Isotype controls should match with the primary antibody species and isotype so that the level of specific staining by the primary antibody may be accurately determined. If using directly labeled primary antibodies, the isotype control works best if conjugated with the same label as the test antibody.

Description: The mouse IgM is useful as an isotype control immunoglobulin. The immunoglobulin was generated by immunization with lipopolysaccharide (LPS). The immunoglobulin is reactive with LPS. Applications Reported: eFluor™ 660 Mouse IgM Isotype Control has been reported for use in flow cytometric analysis and immunohistochemical staining. Applications Tested: eFluor™ 660 Mouse IgM Isotype Control has been tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at the same concentration as the experimental antibody.eFluor™ 660 is a replacement for Alexa Fluor™ 647. eFluor™ 660 emits at 659 nm and is excited with the red laser (633 nm). Please make sure that your instrument is capable of detecting this fluorochome.Excitation: 633-647 nm; Emission: 668 nm; Laser: Red Laser.Filtration: 0.2µm post-manufacturing filtered.
Specifications

Specifications

Mouse IgM
Not Applicable
IgM
Monoclonal
Control
PBS with 0.1% gelatin and 0.09% sodium azide; pH 7.2
RUO
11E10
eFluor 660
Control, Flow Cytometry, Immunohistochemistry
Liquid
Isotype Control
Store at 2-8°C. Do not freeze. Light-sensitive material.

For Research Use Only.

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