IL-10 Rat anti-Human, Functional Grade, Clone: JES3-9D7, eBioscience™ 

Rat Monoclonal Antibody

Manufacturer:  Invitrogen 16710881

Catalog No. 50-147-16

Description

Description: The JES3-9D7 monoclonal antibody reacts with human interleukin-10 (IL-10). Applications Reported: The JES3-9D7 antibody has been reported for use in ELISA capture, ELISPOT capture, neutralization, and intracellular staining for flow cytometric analysis. Applications Tested: The JES3-9D7 antibody has been tested for neutralizing IL-10 binding and biological activity. Endotoxin: Less than 0.001 ng/ug antibody as determined by the LAL assay. Storage and handling: Use in a sterile environment. Filtration: 0.2 µm post-manufacturing filtered. Purity: Greater than 90%, as determined by SDS-PAGE. Endotoxin Level: Less than 0.001 ng/µg antibody, as determined by LAL assay. Aggregation: Less than 10%, as determined by HPLC.

Interleukin 10 (IL-10, CSIF) is an anti-inflammatory cytokine mainly produced by macrophages and Th2 cells. IL-10 is an 18.6 kDa protein of 160 amino acid residues that shares over 80% sequence homology with the Epstein-Barr Virus protein (BCRFI). The reported biological activities of IL-10, which maybe interrelated, include inhibition of macrophage-mediated cytokine synthesis, suppression of the delayed-type hyper-sensitivity response, and stimulation of the Th2 cell response which results in elevated antibody production. IL-10 functions by inhibiting pro-inflammatory cytokines made by macrophages and regulatory T cells including IFN-gamma, TNF-alpha, IL-2, and IL-3, IL-4, and GM-CSF. IL-10 is also known to suppress antigen presentation on antigen presenting cells, enhances B cell survival, proliferation, and antibody production. IL-10 can block NF-kappa B activity, and is involved in the regulation of the JAK-STAT signaling pathway. While human IL-10 can act on murine cells, murine IL-10 cannot act on human cells. Binds to a retinoid X receptor (RXR) responsive element from the cellular retinol-binding protein II promoter (CRBPII-RXRE). IL-10 inhibits the 9-cis-retinoic acid-dependent RXR alpha transcription activation of the retinoic acid responsive element. Knockout studies in mice suggest the function of this cytokine as an essential immunoregulator in the intestinal tract.

Specifications

• Antigen: IL-10
• Classification: Monoclonal
• Concentration: 1 mg/mL
• Formulation: PBS with no preservative; pH 7.2
• Gene Accession No.: P22301
• Gene Alias: Interleukin-10, CSIF, IL-10, IL10A, TGIF
• Host Species: Rat
• Purification Method: Affinity chromatography
• Regulatory Status: RUO
• Type: Antibody
• Monoclonal or Polyclonal: Monoclonal
• Target Species: Human

• Applications: ELISA, Functional Assay, Neutralization
• Clone: JES3-9D7
• Conjugate: Functional Grade
• Gene: IL10
• Format: Liquid
• Gene Symbols: IL10
• Isotype: IgG1, kappa
• Quantity: 50 μg
• Storage Requirements: 4° C
• Primary or Secondary: Primary
• Gene ID (Entrez): 3586

For Research Use Only.