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IL-12/IL-23 p40 Mouse anti-Human, eFluor® 660, Clone: C8.6, eBioscience™ Non-distribution product as customer accommodation.

Mouse Monoclonal Antibody

Manufacturer:  Invitrogen 50712942

Catalog No. 50-112-4439


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Description

Description

Description: The C8.6 antibody reacts with the p40 subunit of human IL-12 p70 and human IL-23. IL-12 is a 70 kDa heterodimeric cytokine composed of two covalently linked chains, p40 and p35, and secreted primarily by activated monocytes, macrophages, and dendritic cells. IL-23 is a biologically-related, heterodimeric cytokine composed of disulfide-linked p40 and p19 subunits. Applications Reported: This C8.6 antibody has been reported for use in intracellular staining followed by flow cytometric analysis. Applications Tested: This C8.6 antibody has been pre-titrated and tested by intracellular staining and flow cytometric analysis of stimulated human cells. This can be used at 5 µL (0.03 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. eFluor® 660 is a replacement for Alexa Fluor® 647. eFluor® 660 emits at 659 nm and is excited with the red laser (633 nm). Please make sure that your instrument is capable of detecting this fluorochome. Excitation: 633-647 nm; Emission: 668 nm; Laser: Red Laser. Filtration: 0.2 µm post-manufacturing filtered.

IL-12 is an interleukin produced by dendritic cells, neutrophils, macrophages and human lymphoblastoid cells as a result of antigenic stimulation. IL-12 is a heterodimeric cytokine encoded by IL-12A (p35) and IL-12B (p40). IL-23 is a heterodimeric cytokine that shares a subunit with IL-12 (IL-12B) along with an IL-23A (IL-23p19) subunit. Functionally, IL-12 increases the expression of vascular endothelial adhesion molecule-1 on the endothelium, directs leukocytes towards tumors and facilitates the ischemic-hemorrhagic necrosis of the tumor tissue. In comparison, IL-23 plays an important role in stimulating memory T-cells, and is required for the induction, expansion, maintece and downstream effector functions of Th17 cells, which play a vital role in upregulating neutrophil chemokines and various pro-inflammatory cytokines.
Specifications

Specifications

IL-12/IL-23 p40
Monoclonal
5 μL/Test
PBS with 0.1% BSA, 0.1% gelatin and 0.09% sodium azide; pH 7.2
P29460
IL12B; Il12p40; interleukin 12B; RP23-388G23.1; Il-12b; Il-12p40; p40; IL-12 p40; IL-23 subunit p40; IL12P40
Mouse
Affinity chromatography
RUO
Antibody
Monoclonal
Human
Flow Cytometry
C8.6
eFluor™ 660
IL12B
Liquid
IL12B
IgG1, kappa
100 Tests
4° C, store in dark, DO NOT FREEZE!
Primary
3593
Documents
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