IL-12 p35 Monoclonal Antibody (C18.2), eBioscience™, Invitrogen™

Rat Monoclonal Antibody

Supplier:  Invitrogen 14712281

Catalog No. 50-136-34

Description

Description: The C18.2 antibody reacts with the p35 subunit of mouse interleukin-12 (IL-12). Interleukin-12 (IL-12) is a heterodimeric 70 kD (p70) cytokine composed of two covalently linked, glycosylated chains, 40kD (p40) and 35-kD (p35). IL-12 is mainly produced by monocytes, macrophages, and dendritic cells in response to bacterial products such as lipopolysaccharides (LPS), to intracellular pathogens or upon interaction with activated T cells. IL-12 was originally discovered because of its ability to induce interferon-gamma (IFN-g) production, cell proliferation, and cytotoxicity mediated by natural killer cells and T cells. It is now established that IL-12 also plays a key role in the development of Th1 responses, leading to IFN-g and IL-2 production. These cytokines can in turn promote T-cell responses and macrophage activation. Applications Reported: This C18.2 antibody has been reported for use in cytokine neutralization, and ELISA capture antibody. Applications Tested: The C18.2 antibody has been tested as the capture antibody in a sandwich ELISA for analysis of bioactive mouse IL-12 p70 in combination with the biotinylated C17.8 (cat. 13-7123) antibody for detection and recombit mouse IL-12 p70 (cat. 14-8121) as the standard. A suitable range of concentrations of this antibody for ELISA capture is 1-4 μg/mL.

Interleukin 12A is a novel signaling cytokine belonging to the Interleukin-6 protein superfamily. Interleukin-12 alpha chain precursor (IL-12A) is a 35-kD subunit of disulfide-linked heterodimer of cytokine IL-12 and has a broad array of biological activities. Reports suggest that IL-12A can act as a growth factor for activated T and NK cells and enhance the lytic activity of NK/lymphokine-activated Killer cells. IL-12A stimulates the production of IFN-gamma by resting PBMC as well as T-cell-independent induction of interferon (IFN)-gamma, and helps in the differentiation of both Th1 and Th2 cells. It is also involved in positive regulation of tyrosine phosphorylation of Stat4 protein. The signaling process of IL-12A in innate immunity requires nitric oxide synthase 2A. Along with EBI3, IL-12A forms a novel heterodimeric cytokine, designated as interleukin-35 (IL-35), that may be specifically produced by T reg cells and is required for maximal suppressive activity.

Specifications

• Antigen: IL-12 p35
• Classification: Monoclonal
• Concentration: 0.5 mg/mL
• Formulation: PBS with 0.09% sodium azide; pH 7.2
• Gene Accession No.: P43431
• Gene Symbols: IL12A
• Purification Method: Affinity chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 16159
• Content And Storage: 4° C
• Form: Liquid

• Applications: ELISA, Functional Assay, Neutralization
• Clone: C18.2
• Conjugate: Unconjugated
• Gene: IL12A
• Gene Alias: CLMF; CLMF p35; cytotoxic lymphocyte maturation factor 1, p35; cytotoxic lymphocyte maturation factor 35 kDa subunit; il 12; IL-12 subunit p35; IL-12, subunit p35; Il12a; IL-12A; IL12p35; IL-12p35; IL35 subunit; ILN; Interleukin; Interleukin 12; interleukin 12 p35 subunit; interleukin 12, p35; interleukin 12a; interleukin 12A (natural killer cell stimulatory factor 1, cytotoxic lymphocyte maturation factor 1, p35); Interleukin12; interleukin-12 alpha chain; interleukin-12 beta p35 subunit; interleukin-12 p35 subunit; interleukin-12 subunit alpha; Ll12a; NF cell stimulatory factor chain 1; NFSK; NK cell stimulatory factor chain 1; NKSF1; P35
• Host Species: Rat
• Quantity: 50 μg
• Primary or Secondary: Primary
• Target Species: Mouse
• Product Type: Antibody
• Isotype: IgG2a κ

For Research Use Only.