IL-15/IL-15R Complex Monoclonal Antibody (GRW15PLZ), Functional Grade, eBioscience™, Invitrogen™

Rat Monoclonal Antibody

Supplier:  Invitrogen 16815682

Catalog No. 50-147-66

Description

Description: The monoclonal antibody GRW15PLZ recognizes the mouse IL-15/IL-15 receptor complex. Interleukin-15 (IL-15) is a 14 kDa proinflammatory protein that is has been shown to play a role in the activation of neutrophils, dendritic cells, and macrophages, and is essential to the development and survival of NK cells and CD8 T-cells. IL-15 activates Jak/Stat pathways by signaling through a heterotrimeric receptor. Two of the subunits of this receptor, beta and gamma, are shared with the IL-2 receptor, while the alpha subunit is unique to IL-15. Despite the expression of IL-15 mRNA in many cell types, the secreted protein is rarely detectable in biological samples. Recent research suggests that IL-15 is retained inside the cell and is only secreted when in complex with its unique receptor, IL-15R alpha. The cytokine/receptor complex may stay bound to the cell surface, where it can be trans-presented to cells expressing the beta gamma subunits of the receptor, or may be secreted in a soluble form that lacks the transmembrane domain of the receptor. The soluble IL-15/IL-15R complex is produced by mouse dendritic cells, fibroblasts, and macrophages and demonstrates a 10-100-fold increase in agonistic activity over IL-15 alone. The monoclonal antibody GRW15PLZ reacts with and inhibits the bioactivity of IL-15 and the IL-15/IL-15R complex. Applications Reported: The monoclonal antibody GRW15PLZ reacts with and inhibits the bioactivity of IL-15 and the IL-15/IL-15R complex.

Interleukin-15 (IL-15) is a 14 kDa proinflammatory protein that has been shown to play a role in the activation of neutrophils, dendritic cells, and macrophages, and is essential to the development and survival of NK cells and CD8 T-cells. IL-15 activates Jak/Stat pathways by signaling through a heterotrimeric receptor. Two of the subunits of this receptor, beta and gamma, are shared with the IL-2 receptor, while the alpha subunit is unique to IL-15.Despite the expression of IL-15 mRNA in many cell types, the secreted protein is rarely detectable in biological samples. Recent research suggests that IL-15 is retained inside the cell and is only secreted in complex with its unique receptor, IL-15R alpha. This chaperoning is required from the ER to Golgi through to secretion. The cytokine/receptor complex may stay bound to the cell surface, where it can be trans-presented to cells expressing the beta/gamma subunits of the receptor, or may be secreted in a soluble form that lacks the transmembrane domain of the receptor. The soluble IL-15/IL-15R complex is produced by mouse dendritic cells, fibroblasts, and macrophages, and demonstrates a 10-100-fold increase in agonistic activity over IL-15 alone.

Specifications

• Antigen: IL-15/IL-15R Complex
• Classification: Monoclonal
• Concentration: 1 mg/mL
• Formulation: PBS with no preservative; pH 7.2
• Gene Accession No.: P48346, Q60819
• Gene Symbols: Il15, Il15ra
• Purification Method: Affinity chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 16168, 16169
• Content And Storage: 4° C
• Form: Liquid

• Applications: Functional Assay, Neutralization
• Clone: GRW15PLZ
• Conjugate: Functional Grade
• Gene: Il15
• Gene Alias: AA690181; AI503618; CD215; Il15; IL-15; IL-15 receptor subunit alpha; Il15ra; IL-15RA; IL-15R-alpha; ILN; Interleukin; interleukin 15; interleukin 15 receptor, alpha chain; interleukin-15; Interleukin-15 receptor subunit alpha; sIL-15 receptor subunit alpha; Soluble interleukin-15 receptor subunit alpha
• Host Species: Rat
• Quantity: 100 μg
• Primary or Secondary: Primary
• Target Species: Mouse
• Product Type: Antibody
• Isotype: IgG1 κ

Safety and Handling

WARNING: Cancer and Reproductive Harm - www.P65Warnings.ca.gov

For Research Use Only.