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IL-15/IL-15R Complex Rat anti-Mouse, Functional Grade, Clone: GRW15PLZ, eBioscience™ Non-distribution product as customer accommodation.

Rat Monoclonal Antibody

Manufacturer:  Invitrogen 16815682

Catalog No. 50-147-66


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Description

Description

Description: The monoclonal antibody GRW15PLZ recognizes the mouse IL-15/IL-15 receptor complex. Interleukin-15 (IL-15) is a 14 kDa proinflammatory protein that is has been shown to play a role in the activation of neutrophils, dendritic cells, and macrophages, and is essential to the development and survival of NK cells and CD8 T-cells. IL-15 activates Jak/Stat pathways by signaling through a heterotrimeric receptor. Two of the subunits of this receptor, beta and gamma, are shared with the IL-2 receptor, while the alpha subunit is unique to IL-15. Despite the expression of IL-15 mRNA in many cell types, the secreted protein is rarely detectable in biological samples. Recent research suggests that IL-15 is retained inside the cell and is only secreted when in complex with its unique receptor, IL-15R alpha. The cytokine/receptor complex may stay bound to the cell surface, where it can be trans-presented to cells expressing the beta gamma subunits of the receptor, or may be secreted in a soluble form that lacks the transmembrane domain of the receptor. The soluble IL-15/IL-15R complex is produced by mouse dendritic cells, fibroblasts, and macrophages and demonstrates a 10-100-fold increase in agonistic activity over IL-15 alone. The monoclonal antibody GRW15PLZ reacts with and inhibits the bioactivity of IL-15 and the IL-15/IL-15R complex. Applications Reported: The monoclonal antibody GRW15PLZ reacts with and inhibits the bioactivity of IL-15 and the IL-15/IL-15R complex.

Applications Tested: The ND50 of GRW15PLZ, as determined by the inhibition of CTLL-2 cell proliferation, is 15-60 ng/mL in the presence of 6 ng/mL recombit mouse IL-15/IL-15R complex, or 0.4-0.8 µg/mL in the presence of 120 ng/mL recombit mouse IL-15. Neutralization dose will vary depending on assay method, cytokine concentration, and cell type. Endotoxin: Less than 0.001 ng/ug antibody as determined by the LAL assay. Storage and handling: Use in a sterile environment. Filtration: 0.2 μm post-manufacturing filtered. Purity: Greater than 90%, as determined by SDS-PAGE. Endotoxin Level: Less than 0.001 ng/μg antibody, as determined by LAL assay. Aggregation: Less than 10%, as determined by HPLC. Interleukin-15 (IL-15) is a 14 kDa proinflammatory protein that has been shown to play a role in the activation of neutrophils, dendritic cells, and macrophages, and is essential to the development and survival of NK cells and CD8 T-cells. IL-15 activates Jak/Stat pathways by signaling through a heterotrimeric receptor. Two of the subunits of this receptor, beta and gamma, are shared with the IL-2 receptor, while the alpha subunit is unique to IL-15.Despite the expression of IL-15 mRNA in many cell types, the secreted protein is rarely detectable in biological samples. Recent research suggests that IL-15 is retained inside the cell and is only secreted in complex with its unique receptor, IL-15R alpha. This chaperoning is required from the ER to Golgi through to secretion. The cytokine/receptor complex may stay bound to the cell surface, where it can be trans-presented to cells expressing the beta/gamma subunits of the receptor, or may be secreted in a soluble form that lacks the transmembrane domain of the receptor. The soluble IL-15/IL-15R complex is produced by mouse dendritic cells, fibroblasts, and macrophages, and demonstrates a 10-100-fold increase in agonistic activity over IL-15 alone.
Specifications

Specifications

IL-15/IL-15R complex
Monoclonal
1 mg/mL
PBS with no preservative; pH 7.2
P48346, Q60819
Il15, Il15ra
IgG1, kappa
100 μg
4° C
Primary
16168, 16169
Functional Assay, Neutralization
GRW15PLZ
Functional Grade
Il15ra
Liquid
Rat
Affinity chromatography
RUO
Antibody
Monoclonal
Mouse
SDS
Documents
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