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Invitrogen™ IL-1R1/IL-1RA Human ELISA Kit

Sandwich ELISA Kit

Manufacturer:  Invitrogen™ EHIL1R1

Encompass

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Catalog No. EHIL1R1


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Description

Description

The Human Interleukin 1 Receptor 1 (Hu IL-1R1/IL-1RA) ELISA quantitates Hu IL-1R1 in human serum, plasma,, or cell culture medium. The assay will exclusively recognize both natural and recombinant Hu IL-1R1. Principle of the method The Human IL-1R1 solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

The protein encoded by this gene is a cytokine receptor that belongs to the interleukin 1 receptor family. This protein is a receptor for interleukin alpha (IL1A), interleukin beta (IL1B), and interleukin 1 receptor, type I(IL1R1/IL1RA). It is an important mediator involved in many cytokine induced immune and inflammatory responses. This gene along with interleukin 1 receptor, type II (IL1R2), interleukin 1 receptor-like 2 (IL1RL2), and interleukin 1 receptor-like 1 (IL1RL1) form a cytokine receptor gene cluster in a region mapped to chromosome 2q12.
Specifications

Specifications

Colorimetric Microplate Reader
96 assays
Other Proteins
Biotin
P14778
6pg/mL to 2000pg/mL
3554
RUO
2°C to 8°C
4 hr. 45 min.
Inflammation
<12%
96 Tests
Plasma, Serum, Supernatant
CD121A,D2S1473,IL-1R-alpha,IL1R,IL1RA,P80
ELISA Kit
This ELISA kit shows no cross-reactivity with the following cytokines tested: human Angiogenin, BDNF, BLC, CNTF, ENA-78, FGF-4, IL-1a, IL-1b, IL-2, IL-3, IL-4, IL-5, IL-6, IL-7, IL-8, IL-9, IL-11, IL-12 p70, IL-12 p40, IL-13, IL-15, IL-309, IP-10, FGF-4, FGF-6, FGF-7, G-CSF, GDNF, GM-CSF, IFN-g, IGFBP-2, IGFBP-3, IGFBP-4, Leptin (OB), MCP-1, MCP-2, MCP-3, MDC, MIF, MIG, MIP-1a, MIP-1 b, MIP-1 delta, PARC, PDGF, RANTES, SCF, SDF-1 alpha, TARC, TGF-b, TIMP-1, TIMP-2, TNF-a, TNF-b, TPO, VEGF.
Solid-phase sandwich ELISA
HRP
8.19-2000 pg/mL
ELISA, Protein Assays, Protein Biology, Cancer Biology, Immunology, Neuroscience, Neurobiology, Protein Detection, Protein Analysis
Pre-coated 96 well plate, Standard, Assay Diluent concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers
Plasma, 50 μL; Serum, 50 μL; Supernatant, 100 μL
1 hr. 20 min.
Human
CD121A, D2S1473, IL-1R-alpha, IL1R, IL1RA, P80
<10%
Human
6 pg/mL
SDS
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