IL-2 Monoclonal Antibody (JES6-5H4), Biotin, eBioscience™, Invitrogen™

Rat Monoclonal Antibody

Supplier:  Invitrogen 13702181

Catalog No. 50-121-51

Description

Description: The JES6-5H4 antibody reacts with mouse interleukin-2 (IL-2), a 17 kDa T cell growth factor and a major immunoregulatory cytokine. Applications Reported: JES6-5H4 has been reported for use in detection of mouse IL-2 by ELISA, intracellular staining and flow cytometric analysis, immunoprecipitation, and for neutralizing IL-2 binding and biological activity. Applications Tested: The JES6-5H4 antibody has been tested as the detection antibody in a sandwich ELISA for analysis of mouse Interleukin-2 (IL-2) in combination with the purified JES6-1A12 (14-7022) antibody for capture and recombinant mouse IL-2 (14-8021) as the standard. A suitable range of concentrations of this antibody for ELISA detection is 0.5-2 μg/mL. A standard curve consisting of doubling dilutions of the recombinant standard over the range of 500 pg/mL - 4 pg/mL should be included in each ELISA plate. Filtration: 0.2 μm post-manufacturing filtered.

Interleukin 2 (IL-2) is an immuno-modulatory cytokine that is important for the proliferation of activated T cells, differentiation of B cells, natural killer cells, monocytes and macrophages. IL-2 signals through the IL-2 receptor (IL-2R), a heterotrimeric protein complex whose gamma chain is also shared by interleukin 4 (IL-4) and interleukin 7 (IL-7). The expression of the IL-2 gene in mature thymocytes is monoallelic, which represents an unusual regulatory mode for controlling the precise expression of a single gene. The targeted disruption of a gene similar to IL-2 in mice leads to an μgcerative colitis-like disease that suggests an essential role of this gene in the immune response to antigenic stimuli.

Specifications

• Antigen: IL-2
• Classification: Monoclonal
• Concentration: 0.5 mg/mL
• Formulation: PBS with 0.09% sodium azide; pH 7.2
• Gene Accession No.: P04351
• Gene Symbols: IL2
• Purification Method: Affinity chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 16183
• Content And Storage: 4° C, do not freeze
• Form: Liquid

• Applications: ELISA, Flow Cytometry, Immunoprecipitation
• Clone: JES6-5H4
• Conjugate: Biotin
• Gene: IL2
• Gene Alias: aldesleukin; cytokine; H-IL-2; il 2; Il2; Il-2; ILN; Interleukin; interleukin 2; interleukin 2 precursor; interleukin 2 variant IL2delta2; Interleukin2; Interleukin-2; interleukin-2 precursor; interleukin-2 precursor protein; interleukin-2; interleukin 2; Involved in regulation of T cell clonal expansion; involved in regulation of T-cell clonal expansion; lymphokine; M-IL-2; patial sequence of Interleukin-2; POIL2; porcine Interleukin 2; T cell growth factor; T-cell growth factor; T-cell growth factor (TCGF); TCGF
• Host Species: Rat
• Quantity: 50 μg
• Primary or Secondary: Primary
• Target Species: Mouse
• Product Type: Antibody
• Isotype: IgG2b κ

For Research Use Only.