IL-21 Monoclonal Antibody (eBio3A3-N2 (3A3-N2)), eFluor™ 660, eBioscience™, Invitrogen™

Mouse Monoclonal Antibody

Supplier:  Invitrogen 50721941

Catalog No. 50-164-65

Description

Description: The monoclonal antibody eBio3A3-N2 reacts with human IL-21, a potent stimulator of NK and cytolytic T cells. IL-21 is a 131 amino acid protein most closely related to IL-2 and IL-15. Expression is found in CD4+ cells upon activation. In addition to its effect on NK cells, IL-21 stimulates proliferation of B-cell stimulated by crosslinking of the CD40 antigen and stimulates the proliferation of bone marrow progenitor cells and the expression of the NK-cell marker CD56 in the presence of IL-15. Applications Reported: This eBio3A3-N2 (3A3-N2) antibody has been reported for use in intracellular staining followed by flow cytometric analysis. Applications Tested: This eBio3A3-N2 (3A3-N2) antibody has been pre-titrated and tested by intracellular staining and flow cytometric analysis of restimulated, Th17-polarized normal human peripheral blood cells. This can be used at 5 μL (0.125 μg) per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. Staining for IL-21 has shown donor variability. stimulation times may vary depending on the donor and should be optimized. Increased staining levels have been observed on Th17-polarized normal human peripheral blood cells. eFluor™ 660 is a replacement for Alexa Fluor™ 647.

IL-21 Monoclonal antibody specifically detects IL-21 in Human samples. It is validated for Flow Cytometry.

Specifications

• Antigen: IL-21
• Classification: Monoclonal
• Concentration: 5 μL/Test
• Formulation: PBS with 0.2% BSA and 0.09% sodium azide; pH 7.2
• Gene Accession No.: Q9HBE4
• Gene Symbols: IL21
• Purification Method: Affinity chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 59067
• Content And Storage: 4° C, store in dark, DO NOT FREEZE!
• Form: Liquid

• Applications: Flow Cytometry
• Clone: eBio3A3-N2 (3A3-N2)
• Conjugate: eFluor 660
• Gene: IL21
• Gene Alias: CVID11; CX2; il 21; IL21; IL-21; ILN; Interleukin; interleukin 21; Interleukin21; interleukin-21; interleukin-21 isoform; porcine interleukin-21; Za11
• Host Species: Mouse
• Quantity: 25 Tests
• Primary or Secondary: Primary
• Target Species: Human
• Product Type: Antibody
• Isotype: IgG1 κ

For Research Use Only.