Description: This 23dcdp monoclonal antibody reacts with the p19 subunit of human IL-23. This heterodimeric cytokine is composed of two disulfide-linked subunits, p40 and p19. It is closely related to IL-12, with which it shares the p40 subunit. The IL-23 receptor is also heterodimeric and shares the IL-12Rbeta1 chain with IL-12, while the IL-23R chain is unique to the IL-23 receptor complex. IL-23R signaling occurs through the Jak/STAT pathway and results in RORgammat expression, which promotes maintece and proliferation of T helper 17 (Th17) cells. Dendritic cells and macrophages produce high levels of p40 and low levels of IL-23 in response to TLR2, TLR4, and TLR8 ligands and agonists of the beta-glucan receptor, Dectin-1. This is due to the presence of monomer and dimer forms of p40, while only a small portion associates with p19 to form IL-23. Our studies suggest donor variability, expression kinetics and type of stimulant can result in variation in IL-23 expression levels. Recent publications suggest the p19 subunit may also exist in the absence of association with p40. Applications Reported: This 23dcdp antibody has been reported for use in intracellular staining followed by flow cytometric analysis. Applications Tested: This 23dcdp antibody has been pre-titrated and tested by intracellular staining and flow cytometric analysis of cultured human dendritic cells. This can be used at 5 µL (0.03 µg) per test.A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. eFluor® is a replacement for Alexa Fluor® 647. eFluor® 660 emits at 659 nm and is excited with the red laser (633 nm). Please make sure that your instrument is capable of detecting this fluorochome. Excitation: 633-647 nm; Emission: 668 nm; Laser: Red Laser. Filtration: 0.2 µm post-manufacturing filtered. This gene encodes a subunit of a cytokine that acts on T and natural killer cells, and has a broad array of biological activities. The cytokine is a disulfide-linked heterodimer composed of the 35-kD subunit encoded by this gene, and a 40-kD subunit that is a member of the cytokine receptor family. This cytokine is required for the T-cell-independent induction of interferon (IFN)-gamma, and is important for the differentiation of both Th1 and Th2 cells. The responses of lymphocytes to this cytokine are mediated by the activator of transcription protein STAT4. Nitric oxide synthase 2A (NOS2A/NOS2) is found to be required for the signaling process of this cytokine in innate immunity.
|4° C, store in dark, DO NOT FREEZE!|
|PBS with 0.1% gelatin, 0.2% BSA and 0.09% sodium azide; pH 7.2|
For Research Use Only.
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