Learn More
IL-23 p19 Monoclonal Antibody (HNU2319), Functional Grade, eBioscience™, Invitrogen™
Mouse Monoclonal Antibody
Supplier: Invitrogen 16523682
Description
Description: The HNU2319 monoclonal antibody is specific to the p19 subunit of human IL-23, a heterodimeric cytokine made up of two covalently linked subunits, p40 and p19. It is closely related to IL-12, with which it shares the p40 subunit. Dendritic cells and macrophages produce IL-23 in response to TLR2, TLR4, and TLR8 ligands, as well as the beta-glucan receptor Dectin-1. Human IL-23 induces proliferation of memory T cells and induces moderate levels of IFN-gamma production by naive and memory T cells, as compared to IL-12. In contrast, the mouse IL-23 biological activities are more distinctly different than those of mouse IL-12 suggesting the signaling or response elements (receptors) differ between species. The IL-23 receptor is also heterodimeric and shares the IL-12Rbeta1 chain with IL-12, while the IL-23R chain is unique to IL-23. Signaling occurs through the Jak/STAT pathway and results in RORgammat expression, which drives the differentiation of CD4^+ T-cells towards the Th17 phenotype. The monoclonal antibody HNU2319 neutralizes the bioactivity of IL-23 without affecting IL-12. Applications Reported: The monoclonal antibody HNU2319 reacts with the p19 subunit of human IL-23 and inhibits its bioactivity. Applications Tested: The ND50 of HNU2319, as determined by the neutralization of mouse IL-17A induction in balb/c splenocytes by recombit human IL-23, is 0.2-0.4 μg/mL in the presence of 2 ng/mL IL-23.
IL-23 is a heterodimeric cytokine composed of the p40 subunit of IL-12 disulfide-linked with a protein p19. p19, like p35 of IL-12, is biologically inactive by itself. IL-23 interacts with IL-12Rbeta1 and an additional, novel beta2-like receptor subunit with STAT4 binding domain, termed IL-23R. IL-23 is secreted by activated mouse and human dendritic cells. Biological activities of mouse IL-23 are distinct from those of mouse IL-12. Mouse IL-23 was found not to induce significant amounts of IFN-γ. Mouse IL-23 does induce strong proliferation of memory T cells (but not naive T cells), whereas IL-12 has no effect on memory cells. Additionally, mouse IL-23 (but not IL-12) can activate mouse memory T cells to produce the proinflammatory cytokine IL-17. Human IL-23 has biological properties which are less distinct from human IL-12; human IL-23 induces proliferation of memory T cells and induces moderate levels of IFN-γ production by naive and memory T cells, as compared to IL-12.Specifications
IL-23 p19 | |
Monoclonal | |
1 mg/mL | |
PBS with no preservative; pH 7.2 | |
Q9NPF7 | |
IL23A | |
Affinity chromatography | |
RUO | |
51561 | |
4° C | |
Liquid |
Functional Assay, Neutralization | |
HNU2319 | |
Functional Grade | |
IL23A | |
il 23; IL 23 A; IL12B; Il-12b; Il12p40; Il-12p40; il23; IL-23; IL-23 subunit alpha; IL23A; IL-23A; IL-23-A; IL23P19; IL-23p19; ILN; Interleukin; interleukin 12B; interleukin 23 p19 subunit; interleukin 23 subunit alpha; interleukin 23, alpha subunit p19; interleukin-23 subunit alpha; Interleukin-23 subunit p19; interleukin-six, G-CSF related factor; JKA3 induced upon T-cell activation; MGC79388; P19; p40; RP23-388G23.1; SGRF; UNQ2498/PRO5798 | |
Mouse | |
100 μg | |
Primary | |
Human | |
Antibody | |
IgG1 κ |
The Fisher Scientific Encompass Program offers items which are not part of our distribution portfolio. These products typically do not have pictures or detailed descriptions. However, we are committed to improving your shopping experience. Please use the form below to provide feedback related to the content on this product.
For Research Use Only.