Promotional price valid on web orders only. Your contract pricing may differ. Interested in signing up for a dedicated account number?
Learn More

Invitrogen™ IL-2RA/CD25 Human ELISA Kit Non-distribution product as customer accommodation.

Sandwich ELISA Kit

$490.00

Specifications

Storage Requirements 2°C to 8°C
Assay Sensitivity 15 pg/mL
Assay Range 16.38-4000 pg/mL
Test Time 1 hr. 20 min.
Sample Volume Plasma, 50 μL; Serum, 50 μL; Supernatant, 100 μL
View More Specs

Products 1
Catalog Number Mfr. No. Quantity Price Quantity & Availability  
Catalog Number Mfr. No. Quantity Price Quantity & Availability  
EHIL2RA Non-distribution product as customer accommodation.
View Documents
Invitrogen™
EHIL2RA
96 Tests
Pack for $490.00
Add to cart
 
Description

Description

The Human Interleukin 2 Receptor Alpha/CD25 (Hu IL-2RA/CD25) ELISA quantitates Hu IL-2RA in human serum, plasma, or cell culture medium. The assay will exclusively recognize both natural and recombinant Hu IL-2RA. Principle of the method The Human IL-2RA solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

CD25 (IL2 receptor alpha chain/IL2RA) is a cytokine that plays a role in the proliferation of T and B lymphocytes. The receptor of this cytokine (IL2RA) is a heterotrimeric protein complex with a gamma chain also shared by interleukin 4 (IL4) and interleukin 7 (IL7). IL2RA, IL2R beta chain (IL2RB), and the IL2R gamma chain (IL2RG), constitute the high-affinity IL2 receptor. Homodimeric IL2RA chains result in low-affinity receptor, while homodimeric IL2RB chains produce a medium-affinity receptor. The expression of IL2 in mature thymocytes is monoallelic, which represents an unusual regulatory mode for controlling the precise expression of a single gene. IL2 is primarily produced by mature T cells. IL2 plays an important role as a growth factor, differentiation factor, and regulator of cell death. IL-2 stimulates the proliferation of B cells, augments natural killer cell activity, and inhibits granulocyte macrophage colony formation. The targeted disruption of a similar gene in mice leads to ulcerative colitis-like disease, which suggests a role in the immune response to antigenic stimuli. Mutations in this gene are associated with interleukin 2 receptor alpha deficiency.
Specifications

Specifications

2°C to 8°C
16.38-4000 pg/mL
Plasma, 50 μL; Serum, 50 μL; Supernatant, 100 μL
<10%
15 pg/mL
1 hr. 20 min.
<12%
SDS
Product Certifications
Provide Content Correction

The Fisher Scientific Encompass Program offers items which are not part of our distribution portfolio. These products typically do not have pictures or detailed descriptions. However, we are committed to improving your shopping experience. Please use the form below to provide feedback related to the content on this product.

Product Title

By clicking Submit, you acknowledge that you may be contacted by Fisher Scientific in regards to the feedback you have provided in this form. We will not share your information for any other purposes. All contact information provided shall also be maintained in accordance with our Privacy Policy.

Cancel Submit