IL-3 beta Polyclonal Antibody, PeproTech®, Invitrogen™

Goat Polyclonal Antibody

Supplier:  Pepro Tech Inc 500P177G50UG

Catalog No. 500P177G50

Description

AA Sequence of recombinant protein: MISDRGSDAH HLLRTLDCRT IALEILVKLP YPQVSGLNNS DDKANLRNST LRRVNLDEFL KSQEEFDSQD TTDIKSKLQK LKCCIPAAAS DSVLPGVYNK DLDDFKKKLR FYVIHLKDLQ PVSVSRPPQP TSSSDNFRPM TVEC. Preparation: Produced from sera of goats immunized with highly pure Recombinant Rat IL-3beta. Anti-Rat IL-3beta-specific antibody was purified by affinity chromatography employing an immobilized Rat IL-3beta matrix. Sandwich ELISA: To detect Rat IL-3beta by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5-2.0 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with PeproTech Biotinylated Anti-Rat IL-3beta (500-P177GBt) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant Rat IL-3beta. Western Blot: To detect Rat IL-3beta by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant Rat IL-3beta is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.

IL-3 beta Polyclonal antibody specifically detects IL-3 beta in Rat samples. It is validated for ELISA, Western Blot

Specifications

• Antigen: IL-3 beta
• Classification: Polyclonal
• Formulation: PBS with no preservative
• Gene Accession No.: P04823
• Immunogen: E.coli-derived Recombinant Rat IL-3beta
• Quantity: 50 μg
• Primary or Secondary: Primary
• Target Species: Rat
• Form: Lyophilized

• Applications: ELISA, Western Blot
• Conjugate: Unconjugated
• Gene: Il3
• Host Species: Goat
• Purification Method: Antigen affinity chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 24495
• Content And Storage: -20°C