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IL-33R (ST2) Rat anti-Mouse, PerCP-eFluor™ 710, Clone: RMST2-2, eBioscience™ Non-distribution product as customer accommodation.

Rat Monoclonal Antibody

$143.00 - $361.00

Specifications

Antigen IL-33R (ST2)
Clone RMST2-2
Target Species Mouse
Conjugate PerCP-eFluor™ 710
Isotype IgG2a, kappa
View More Specs

Products 2
Catalog Number Mfr. No. Quantity Price Quantity & Availability  
Catalog Number Mfr. No. Quantity Price Quantity & Availability  
50-112-8826 Non-distribution product as customer accommodation. View Documents Invitrogen
46933580
25 μg Each for $143.00
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50-112-3946 Non-distribution product as customer accommodation. View Documents Invitrogen
46933582
100 μg Each for $361.00
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Description

Description

Description: The RMST2-2 monoclonal antibody reacts with ST2, also known as IL-33 receptor (IL-33R) or interleukin 1 receptor-like 1. ST2 is a member of the IL-1 receptor family. In the membrane-bound form, it consists of three extra-cellular immunoglobulin domains and an intracellular toll-interleukin-1 receptor domain. ST2 forms a dimer with IL-1R accessory protein in a ligand-dependent manner. The IL-33/ST2 interaction induces the production of TH2 cytokines. ST2 also has a soluble isoform lacking transmembrane and intracellular toll-interleukin-1 receptor domains. It is believed that the soluble form functions as a decoy receptor that can block membrane bound IL-33/ST2 interaction. ST2 is expressed by TH2 lymphocytes, mast cells, eosinophils, basophils, innate lymphocytes, smooth muscle cells, and endothelial cells and is involved in host defense, allergy, and inflammation. The IL-33/ST2 interaction has also been shown to be atheroprotective. Applications Reported: This RMST2-2 antibody has been reported for use in flow cytometric analysis. Applications Tested: This RMST2-2 antibody has been tested by flow cytometric analysis of mouse bone marrow-derived mast cells. This can be used at less than or equal to 0.25 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.

It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. PerCP-eFluor® 710 emits at 710 nm and is excited with the blue laser (488 nm); it can be used in place of PerCP-Cyanine5.5. We recommend using a 710/50 bandpass filter, however, the 695/40 bandpass filter is an acceptable alternative. Please make sure that your instrument is capable of detecting this fluorochrome. Fixation: Samples can be stored in IC Fixation Buffer (cat. 00-8222) (100 µL cell sample + 100 µL IC Fixation Buffer) or 1-step Fix/Lyse Solution (cat. 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically. Excitation: 488 nm; Emission: 710 nm; Laser: Blue Laser. Filtration: 0.2 µm post-manufacturing filtered. ST2 is a member of the interleukin 1 receptor family. Studies of ST2 in mouse suggested that this receptor can be induced by proinflammatory stimuli, and may be involved in the function of helper T cells. ST2 belongs to the interleukin-1 receptor family with three Ig-like C2-type (immunoglobulin-like) domains and a TIR domain. ST2 is a receptor for interleukin-33 (IL-33) which upon stimulation recruits the signaling molecules MYD88, IRAK1, IRAK4, and TRAF6, followed by phosphorylation of MAPK3/ERK1 and/or MAPK1/ERK2, MAPK14, and MAPK8. Reports also suggest its possible role in helper T-cell function as well as in cell growth. Three isoforms of ST2 have been reported where isoform C is a membrane form and isoform B is a secreted form. ST2 may be induced by proinflammatory stimuli, and may also be found in serum of autoimmune disease. Different isoforms are restricted to different tissues while high expression of this protein is evident in kidney, lung, placenta, stomach, skeletal muscle, colon and small intestine.
Specifications

Specifications

IL-33R (ST2)
Mouse
IgG2a, kappa
Monoclonal
Liquid
Antibody
4° C, store in dark, DO NOT FREEZE!
RMST2-2
PerCP-eFluor™ 710
Flow Cytometry
Primary
Monoclonal
PBS with 0.1% gelatin and 0.09% sodium azide; pH 7.2
RUO

For Research Use Only.

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